摘要
目的探讨三黄糖肾康颗粒对2型糖尿病大鼠骨质血清淀粉样蛋白A表达的影响。方法将75只3月龄雌性wistar大鼠,采用随机数字法分为对照组10只、糖尿病模型组65只,糖尿病模型组按血糖水平随机分糖尿病组、中药预防组、中药高剂量组、中药低剂量组和西药治疗组各10只,取材后测空腹血糖、骨密度和骨组织A-SAAm RNA、蛋白表达水平。结果与糖尿病模型组比较,除正常对照组外,其余组A-SAAm RNA、蛋白表达水平和血糖下降,中药低剂量组骨密度上升,差异均有统计学意义(P<0.05)。结论血清淀粉样蛋白A可能是导致2型糖尿病骨质疏松的一个重要原因,通过抑制血清淀粉样蛋白A,三黄糖肾康颗粒对治疗2型糖尿病骨质疏松具有确切疗效。
Objective： To investigate the effect of Sanhuang Tangshenkang granule on on the expression of A-SAA in rats with type 2 diabetes mellitus complicated with osteoporosis. Methods： Seventy-five three months old female rats were randomly divided into control group （n = 10） and diabetic model group （n = 65）. The diabetic model group was randomly divided into three groups according to the blood glucose level, the traditional Chinese medicine preven- tion group, the traditional Chinese medicine high dose group, the low dose group and the western medicine treatment group. The expression of A-SAA mRNA and protein in fasting blood glucose, bone mineral density and bone tissue were measured. Results： Compared with the control group, the levels of A-SAA mRNA, protein and blood glucose were decreased in the other groups except the normal control group, and the bone mineral density of the low dose group was higher.（P〈0.05）. Conclusion： Serum amyloid A may be an important cause of osteoporosis in type 2 diabetes mellitus by inhibiting serum amyloid A. Sanhuang Tangshenkang granule has a definite effect on the treatment of osteoporosis in type 2 diabetes mellitus.
出处
《中医药临床杂志》
2017年第5期647-649,共3页
Clinical Journal of Traditional Chinese Medicine
基金
辽宁省自然科学基金(201202147)
