摘要
目的:通过观察中药复方益气调脂八味汤对载脂蛋白E基因敲除(ApoE^(-/-))小鼠主动脉粥样硬化斑块病理形态和CD40-CD40L系统信号的影响,探讨益气调脂八味汤抗动脉粥样硬化的作用机制。方法:40只8周龄雄性ApoE^(-/-)小鼠予高脂饲料喂养建立实验性动脉粥样硬化小鼠模型,至第12周龄时,ApoE^(-/-)小鼠随机分为模型对照组、氯吡格雷组、中药高剂量组和低剂量组。取10只8周龄近交系C57BL/6小鼠作为正常对照组给予普通饲料喂养。各组于第24周取材检测血脂、外周血血小板表面PAC-1、CD40L表达;观察主动脉组织病理形态学改变;检测主动脉组织CD40L表达水平。结果:与模型对照组比较,中药2个剂量组小鼠血清TC、TAG和LDL-C水平均降低,HDL-C水平均升高,差异有统计学意义;与高剂量组比较,低剂量组小鼠血清TC、TAG、LDL-C和HDL-C水平比较差异均无统计学意义。中药组病变程度明显轻于模型对照组,并较氯吡格雷组轻。与模型对照组比较,中药个剂量组小鼠血清PAC-1、CD40L表达的百分比均明显降低,差异有统计学意义;与高剂量组比较,低剂量组小鼠血清PAC-1、CD40L表达百分比差异均无统计学意义。模型组动脉组织CD40L表达光密度值明显高于正常对照组;与高剂量组相比,低剂量组CD40L平均光密度值比较差异无统计学意义。结论:中药复方益气调脂八味汤能够调节血脂,调控CD40-CD40L系统的信号表达而抑制血小板活化,具有明显的抗动脉粥样硬化作用,且不存在剂量差异。
Objective： To observe the effects of Chinese medicine Yiqi Tiaozhi Baiwei Decoction on ApoE^（-/-）mice atherosclerotic plaques and pathological signal CD40-CD40 L system and research the mechanism of anti artery atherosclerosis. Methods： Fourty 8-week-old male ApoE^（-/-）mice were fed high-fat diet to build experimental model of atherosclerosis. When 12 weeks of age,ApoE^（-/-）mice were randomly divided into model group,clopidogrel Gray group,high dose group and low dose group. Ten 8-week-old inbred C57BL/6 mice were used as normal control group and were given normal diet. At 24 th week,each group＇s blood lipid,peripheral blood platelet surface PAC-1,CD40 L expression,aortic pathological changes,aortic CD40 L expression levels were detected and compared. Results： Compared with the model group,Chinese medicine both dose groups＇ serum TC,TAG and LDL-C levels were reduced,HDL-C increased and the difference was statistically significant. Compared with the high dose group,the low dose group＇s serum TC,TAG,LDL-C and HDL-C levels were not statistically significant. Medicines severity was significantly lighter than the model control group＇s and lighter than the clopidogrel group. Compared with the model group,Chinese medicine both dose groups＇ serum PAC-1 and the percentage of CD40 L expression were significantly lower. The difference was statistically significant.Compared with the high dose group,the low dose group＇s serum PAC-1 and CD40 L expression percentage were not statistically significant. Model＇s arterial tissue optical density CD40 L expression was significantly higher than the control group＇s. Compared with high dose group,low dose group ＇s CD40 L mean optical density value was not statistically significant.Conclusion： Chinese medicine Yiqi Tiaozhi Baiwei Decoction can regulate blood lipids,the expression of CD40-CD40 L signal system and inhibit platelet activation,with a clear anti-atherosclerotic effect and there is no difference in dose.
出处
《中华中医药学刊》
CAS
北大核心
2017年第6期1509-1512,I0028,I0029,共6页
Chinese Archives of Traditional Chinese Medicine
基金
河南省中医药科学研究专项项目(2014ZY02104)
