摘要
目的探究射干麻黄汤对哮喘大鼠嗜酸粒细胞（eosinophilia,EOS）浸润、凋亡及死亡受体（factor associated suicide,Fas）和B细胞淋巴瘤-2（B cell lymphoma-2,Bcl-2）表达的影响。方法选取荷兰种健康大鼠40只作为研究对象,分为对照组、哮喘模型组、地塞米松组、射干麻黄汤组四组,每组10只,采用卵蛋白反复激发法制备大鼠哮喘模型,对各组大鼠哮喘症状进行评分,染色法检测肺组织中EOS浸润数,四甲基偶氮唑盐（methyl thiazolyl tetrazolium,MTT）法检测EOS凋亡,Western blot检测Fas、Bcl-2蛋白的表达;流式细胞仪检测大鼠EOS凋亡。结果与模型组大鼠比较,地塞米松组、射干麻黄汤组大鼠哮喘症状均明显减轻,评分亦明显降低（P〈0.05）,地塞米松组与射干麻黄汤组对比,差异无统计学意义。哮喘模型组静脉血以及肺组织中EOS浸润数、EOS%均明显高于对照组,EOS凋亡率明显低于对照组（P〈0.05）。地塞米松组、射干麻黄汤组EOS浸润数、EOS%均明显低于模型组仍高于对照组,EOS凋亡率明显高于模型组与对照组（P〈0.05）,地塞米松组与射干麻黄汤组对比,差异无统计学意义。地塞米松组、射干麻黄汤组Fas蛋白表达量明显高于对照组以及模型组（P〈0.05）,Bcl-2蛋白明显低于对照组以及模型组（P〈0.05）,地塞米松组与射干麻黄汤组对比,差异无统计学意义。细胞流式仪检测结果显示,对照组细胞无细胞凋亡现象,经射干麻黄汤处理后,细胞出现大量凋亡。结论射干麻黄汤能够诱导EOS凋亡,可能与上调Fas表达,下调Bcl-2表达相关。
Objective To explore the infiltration and apoptosis of eosinophilia（EOS）and the influence of death receptor（Fas） and B cell lymphoma-2 （Bcl-2） expression after Belamcanda and Ephedra Decoction on asthma rats.Methods40 healthy rats of Holland were selected as the research subjects,they were divided into four groups： control group,asthma model group, dexamethasone group,Belamcanda and Ephedra Decoction group with 10 rats in each group.Rat asthma model was prepared by repeated stimulation of egg protein,the symptoms of asthma in each group were evaluated;the number of EOS infiltration in lung tissue was detected by staining method;EOS apoptosis was detected by methyl thiazolyl tetrazolium（MTT） method;Blot Western was used to detect the expression of Fas and Bcl-2 protein;the flow cytometry was used to detect the apoptosis of EOS in rat.ResultsCompared with the rats in the model group, dexamethasone group, Belamcanda and Ephedra Decoction group rats asthma symptoms were significantly reduced, scores were significantly lower （P〈 0.05）,there was no significant difference between Dexamethasone group and sheganmahuang Decoction group.In the asthma model group,the number of EOS infiltration and EOS% in venous blood and lung tissue were significantly higher than those in control group,EOS apoptosis rate was significantly lower than that of the control group （P〈 0.05）.In Dexamethasone group,Belamcanda and Ephedra Decoction group infiltration number of EOS and EOS% were significantly lower than that of model group but higher than the control group, the apoptosis rate of EOS protein was significantly higher than the model group and the control group （P〈 0.05）,there was no significant difference between Dexamethasone group and sheganmahuang Decoction group.The expression of Fas in dexamethasone group,Belamcanda and Ephedra Decoction group was significantly higher than the control group and model group （P〈 0.05）,Bcl-2 protein was significantly lower than the control group and model group （P〈 0.05）,there was no significant difference between Dexamethasone group and sheganmahuang Decoction group.Cell flow cytometry showed that the control group had no cell apoptosis,a large number of apoptotic cells were appeared after Belamcanda and Ephedra Decoction treat with.ConclusionBelamcanda and Ephedra decoction can induce apoptosis of EOS expression,it may be associated with the upregulation of Fas, downregulation of Bcl-2 expression.
作者
刘月波
金湛
章小敏
王爱飞
何昱
LIU Yue-bo JING Zhan ZHANG Xiao-min WANG Ai-fei HE Yu(Department of Traditional Chinese Medicine, Zhejiang Chinese Medicine University, Hangzhou 310053, China Department of Traditional Chinese Medicine, Three Hospital of Traditional Chinese Medicine, Taizhou 317100, China Department of Traditional Chinese Medicine, Three People＇s Hospital of Zhejiang, Taizhou 317100, China)
出处
《中国生化药物杂志》
CAS
2017年第5期10-13,共4页
Chinese Journal of Biochemical Pharmaceutics
