摘要
目的:观察地塞米松(DM)对哮喘豚鼠体外不同密度嗜酸性粒细胞(Eos)凋亡及fas、bcl-2mRNA表达的影响,探讨DM促进哮喘Eos凋亡的机制。方法:卵蛋白激发哮喘豚鼠动物模型48h后行支气管肺泡灌洗,分离低密度及正常密度Eos(HEos,NEos)。HEos及NEos分别与地塞米松(DM)(10-10-10-5mol/L)共培养24h,以原位杂交方法检测不同Eos的fas及bcl-2mRNA表达,3’末端脱氧核苷转移酶介导的脱氧三磷酸尿苷(d-UTP)缺口末端标记(TUNEL)法检测细胞凋亡。结果:DM干预24h后,可见Eos细胞凋亡增加的同时,不同密度Eos的fasmRNA表达也增加,bcl-2mRNA表达降低,并与DM浓度呈剂量依赖性。fas表达与Eos凋亡呈正相关(P<0.05),而bcl-2的表达与Eos凋亡呈负相关(P<0.05)。结论:DM可促进不同密度Eosfas表达,抑制其bcl-2表达,促进Eos凋亡。DM可以通过嗜酸性粒细胞fas表达增加及bcl-2表达减少而调节Eos凋亡。
AIM： To investigate the effects of dexamethasone （DM） on expression of f as and bcl - 2 mRNA and apoptosis in eosinophils （Eos） of bronchoalveolar lavage fluid （BALF） in asthmatic guinea pig. METHODS： The guineas pigs were stimulated with ovalbumin （OVA） for48 hours. Hypedense Eos （HEos） and normodense Eos （NEos） were purified from BALF by gradients of Percoll. Eos was cultured in PRMI- 1640 medium and DM （10^-10- 10^-5mol/L） was added. The mRNA of f as and bcl - 2 in Eos was measured by hybridization. Apoptosis was detected by TdT- mediated dUTP nick end labeling （TUNEL）. RESULTS： Eos was cultured under the condition of DM administration in vitro. Apoptotic Eos increased, fas mRNA expression increased and the bcl - 2 mRNA expression decreased in a dose - dependent manner. There was significant correlation between Eos apoptosis and fas mRNA expression. The expression of bcl - 2 mRNA was inversely correlated with Eos apoptosis. CONCLUSION： DM promoted Eos apoptosis, increased expression of fas and inhibited expression of bcl - 2 in a dose - dependent manner in vitro, fas and bcl - 2 might be one of mechanisms of DM promoting apoptosis in Eos.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2006年第3期577-580,共4页
Chinese Journal of Pathophysiology
