摘要
目的建立HPLC法同时测定当飞利肝宁胶囊(水飞蓟和当药)中獐牙菜苦苷、龙胆苦苷、当药醇苷、花旗松素、水飞蓟亭、水飞蓟宁、水飞蓟宾的含有量。方法该药物75%甲醇提取液的分析采用Kromasil C18色谱柱(4.6 mm×250 mm,5.0μm);以甲醇-0.1%甲酸为流动相,梯度洗脱;体积流量0.9 mL/min;柱温30℃;检测波长254 nm和288 nm。结果 7种成分在各自范围内线性关系良好(r>0.999 0),平均加样回收率96.78%~100.45%,RSD均小于2.0%。结论该方法快速准确,重复性好,可用于当飞利肝宁胶囊的质量控制。
AIM To establish an HPLC method for the simultaneous content determination of swertiamarin,gentiopicrin,swertianolin,taxifolin,silychristin,silydianin and silybin in Dangfei Liganning Capsules（Silybi Fructus and Swertiae Herba）.METHODS The analysis of 75% methanol extract of this drug was performed on a30 ℃ thermostatic Kromasil C18column（4.6 mm×250 mm,5.0 μm）,with the mobile phase comprising of methanol-0.1% formic acid flowing at 0.9 mL/min in a gradient elution manner,and the detection wavelengths were set at 254 nm and 288 nm.RESULTS Seven constituents showed good linear relationships within their own ranges（r〉0.999 0）,whose average recoveries were 96.78%-100.45% with the RSDs of less than 2.0%.CONCLUSION This accurate and reproducible method can be used for the rapid quality control of Dangfei Liganning Capsules.
出处
《中成药》
CAS
CSCD
北大核心
2017年第6期1187-1190,共4页
Chinese Traditional Patent Medicine
关键词
当飞利肝宁胶囊
化学成分
HPLC
Dangfei Liganning Capsules
chemical constituents
HPLC
