摘要
目的：建立水飞蓟宾葡甲胺在犬体内血药浓度的高效液相色谱检测方法．方法：在弱碱性条件下．血样用叔丁基甲醚进行液-液提取．结果：犬血浆中的成分对水飞蓟宾葡甲胺的检测没有干扰，且水飞蓟宾葡甲胺异构体得到很好分离。线性范围为0．1～5．0μg·mL^-1，高中低3个浓度的平均绝对回收率为（94．2±6．12）％，方法回收率为（104．6±11．05）％。结论：该方法简单准确，灵敏度高，适用于水飞蓟宾葡甲胺体内药动学及临床药理学研究．
AIM： To establish an RP-HPLC method for the determination of silybin meglumine in dog plasma. METHOD： To extract silybin meglumine by methyl t-butyl ether from the plasma in the weak base solution. RESULTS： Silvbin meglumine was separated with no interference found and the two isomers were separated finely. A good linear relationship was obtained between 0.1 - 5.0 μg· mL^-1 （ r = 0.9972）. The average absolute recovery of low, medium and high concentration was （94.2 ± 6.12） %. The average method recovery was （104.6 ± 11.05） %. CONCLUSION： This method is simple, sensitive and accurate. It can be widely used in the pharmacokinetie and clinical research of silybin meglumine.
出处
《中国天然药物》
SCIE
CAS
CSCD
2006年第3期207-209,共3页
