摘要
目的:饮片质量均一性是中药临床疗效稳定的基础,利用化学指纹图谱结合DNA分子鉴定技术考察三七精准煮散饮片与市售原饮片质量的均一性。方法:应用第二内转录间隔区(ITS2)序列DNA条形码对三七饮片进行分子鉴定;对比三七市售原饮片及精准煮散饮片的干膏收率;采用HPLC-DAD检测3批三七饮片混合粉碎前后的质量均一性和指纹图谱相似度。结果:三七煮散饮片出膏率显著高于原饮片出膏率;不同批次原饮片间指标成分三七皂苷R_1,人参皂苷Rg_1及Rb_1溶出量有明显的差异性,RSD分别为13.1%,32.7%及24.5%,混合制成煮散饮片后溶出量差异减小,RSD分别为3.6%,2.6%及3.5%;精准煮散饮片提取液中化学成分溶出量较原饮片高。混合粉碎后指纹图谱相似度提高,标定了共有峰14个,峰面积均有提高。结论:三七精准煮散饮片与市售原饮片基本属性相一致,但煮散饮片的浸出、有效成分溶出及质量均一性均有明显提高,提示煮散饮片可提高临床用药的精准性。
Objective:Clinical curative effect is closely related to the specifications and quality of Chinese medicine decoction pieces.Chemical fingerprint methods and DNA molecular identification technology were used to evaluate the quality of precise powder decoction pieces(PPDP)of Notoginseng Radix et Rhizoma by comparing with the traditional commercial slices.Method:Different specifications of PPDP of Notoginseng Radix et Rhizoma were prepared,their dry extract yields were in contrast with those of commercial slices;three hatches of commercial slices were collected,the content uniformity,fingerprint and similarity evaluation before and after the mixing and pulverization were studied by HPLC-DAD and DNA sequence alignment.Result:Dry extract yield of PPDP of Notoginseng Radix et Rhizoma were remarkablely higher than that of the traditional commercial slices.Dissolution rates of notoginsenoside R1,ginsenoside Rg1 and RbI in PPDP were higher than those of traditional commercial slices.RSDs of inter-assay dissolution of the three components of commercial slices were 13.1%,32.7%and 24.5%,respectively,which could be reduced to 3.6%,2.6%and 3.5%after mixing and preparing into PPDP.After comminution,the similarity of fingerprints was improved,14 peaks were identified and their peak areas were improved.Conclusion:PPDP and traditional commercial slices of Notoginseng Radix et Rhizoma share consistent properties,while PPDP can apparently improve dry extract yield,dissolution rate and quality uniformity.
作者
张靖
雷迪
丘小惠
黄志海
徐文
李西文
Julie A.Hawkins
陈士林
ZHANG Jing;LEI Di;QIU Xiao-hui;HUANG Zhi-hai;XU Wen;LI Xi-wen;Julie A.Hawkins;CHEN Shi-lin(The Second Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangdong Provincial Academy of Chinese Medical Sciences,Guangdong Branch of China Academy of Chinese Medical Sciences,Guangzhou 510006,China;School of Biological Science,University of Reading,Reading 999020,United Kingdom;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2017年第11期13-17,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广东省科技厅-广东省中医药科学院联合专项(2014A020221101)
广东省中医院院内专项(2015KT1817)
中国中医科学院中医药健康服务发展专项(ZZ0908067)
关键词
三七
精准煮散饮片
DNA条形码
指纹图谱
三七皂苷类
人参皂苷类
Notoginseng Radix et Rhizoma
precise powder decoction pieces
DNA barcode
fingerprints
saponins from Notoginseng Radix et Rhizoma
ginsenosides
