摘要
青霉素废菌渣是制药工业青霉素发酵工序产生的固体废弃物,处理不当会导致环境污染、危及生态。本文以废菌渣为原料,在碱性条件下,采用微波水解方法得到复合氨基酸。将其作为石膏缓凝剂,可以实现废菌渣的安全、高附加值利用。在单因素实验的基础上,以碱浓度、料液比、反应时间、反应温度为考察因素,采用正交实验法确定最佳工艺条件为:碱浓度0.04mol/L,料液比1∶4,微波水解时间10min,温度85℃。借助氨基酸分析仪分析了水解产物的化学组成,利用红外光谱、扫描电镜等手段对缓凝作用(石膏体系)机理进行了分析研究。结果表明:水解物主要是赖氨酸、酪氨酸、半胱氨酸等氨基酸的混合物,其中氨基和羧基官能团参与了钙的配位,形成了钙的复合氨基酸/小肽螯合物,从而延缓了石膏的溶解、水化和结晶过程,使凝结时间延长。
Waste penicillin mycelium （WPM） is solid waste from the fermentation process of penicillin in pharmaceutical industry, which will result in air pollution and threaten the ecological environment. WPM was treated by microwave hydrolysis in alkali condition to produce mixed amino acids, which can be used for gypsum retarder to realize safe utilization of WPM. Based on the single factor experiment, four factors were selected for orthogonal experiment： alkali concentration, solid-liquid ratio, treatment time, and temperature. The optimal conditions for the hydrolysis process were obtained： alkali concentration 0.04mol/L, solid-liquid ratio 1 ： 4, reaction time 10min, and at 85 ℃. Chemical composition of the product was analyzed using high speed amino acid analyzer. The retarding mechanism for desulphurization gypsum was investigated using FTIR and SEM. The results showed that the retarder contained amino acids such as lysine, tyrosine, and cysteine, etc. The amino and carboxyl group in the retarder chelated with calcium ion, forming mixed amino acids/small peptides complexes, which delayed dissolution, hydration and crystallization of gypsum crystals, thereby prolonging the setting time of gypsum paste.
出处
《化工进展》
EI
CAS
CSCD
北大核心
2017年第6期2275-2281,共7页
Chemical Industry and Engineering Progress
基金
国家自然科学基金项目(21304030)
关键词
废菌渣
石膏缓凝剂
微波
蛋白质
水解
复合氨基酸
肽
mycelium residue
gypsum retarder
microwave
protein
hydrolysis
mixed amino acids
petptide
