摘要
利用碳二亚胺法合成2,4-二氯苯氧乙酸(2,4-D)的包被抗原,用高碘酸钠法制备了辣根过氧化物酶标记的2,4-D抗体,随后用包被原包被酶标板并建立了快速准确检测豆芽样品中2,4-D残留的直接竞争酶联免疫吸附分析(ELISA)法。结果表明:包被抗原与酶标记抗体的最佳稀释比例分别为1:160和1:32000。该方法的线性范围为(2~250)ng/mL(R^2=0.997),检测限为0.63 ng/mL,在该范围其相关性良好。其日内和日间的变异系数(CV)分别在3.03%~7.58%和2.81%~6.87%之间,均小于10%,精密度良好。平均回收率为95.5%,有较高的准确度和良好的特异性。因此,该直接竞争ELISA法可以快速准确地实现对豆芽中残留的2,4-D检测。
The coating antigen of the 2,4-dichlorophenoxyacetic （2,4-D） and the horseradish peroxidase （HRP）-Iabeled anti-2,4-D antibody （anti-2,4-D-HRP） were prepared by the carbodiimide crosslinking method and sodium periodate method, respectively. A direct competitive enzyme-linked immunosorbent assay （ELISA） was developed for the fast and precise detection of residual 2,4-D in bean sprouts samples with ELISA plates incubated by coating antigen. The results showed： the best dilution ratios of coating antigen and anti-2,4-D-HRP were 1：160 and 1：32000, respectively. The linear range of this method was from 2 ng/mL to 250 ng/mL （R2=0.997） and the limit of detection （LOD） was 0.63 ng/mL. The precision of the method is good with the coefficient of variations （CV） of intra-day and inter-day were from 3.03% to 7.58% and 2.81% to 6.87%, respectively, which were both low 10%. The specificity was satisfactory and the mean recovery was 95.5%, so the precise was good. Therefore, this direct competitive ELISA could complete the fast and precise detection of residual 2,4-D in bean sprouts samples.
出处
《食品科技》
CAS
北大核心
2017年第5期290-295,共6页
Food Science and Technology
基金
国家自然科学基金项目(21572219)
四川省2015大学生创新训练项目(201513705078)
四川省教育厅科研计划项目(16ZA0299)
关键词
2
4-二氯苯氧乙酸
直接竞争ELISA
豆芽
残留检测
2,4-dichlorophenoxyacetic
direct competitive enzyme-linked immunosorbent assay
bean sprout
residue detection
