摘要
旨在了解掌握乌鲁木齐地区屠宰的绵羊群戊型肝炎流行情况。采用双抗原夹心酶联免疫法(DS-ELISA)和免疫组化法分别对来自新疆6个县(市)的258份屠宰绵羊血清中HEV抗体和229份肝脏样品中HEV抗原进行了检测,对不同地区、不同年龄、不同品种绵羊HEV感染率进行了比较。结果显示,屠宰绵羊血清HEV抗体阳性率为14.7%(38/258),肝脏HEV抗原感染率为14.0%(32/229),不同地区之间感染率差异显著(P<0.05);无论检测血清样品,还是检测肝脏样品,公羊的感染率(16.7%和15.0%)均高于母羊的感染率(13.7%和13.4%);所检测的6个品种绵羊中均存在HEV感染,其中吐鲁番地区饲养的黑羊感染率最高,血清抗体感染率为17.1%,肝脏HEV感染率为17.2%;不同年龄的绵羊感染率之间有一定的差异,1岁以下的绵羊与2岁以上的绵羊感染率相比差异极显著(P<0.01)。透射电镜观察显示,肝脏样品出现肝细胞变性、细胞核挤于细胞边缘、细胞核周围出现空泡、细胞核萎缩、空泡占满细胞质、细胞器减少等病理变化。研究结果说明新疆地区绵羊仍然普遍存在HEV感染。
To investigate the epidemic situation of hepatitis E in the slaughtered sheep in Urumqi area, double antigen sandwich ELISA （DS-ELISA） and immunohistochemistry were respectively used to detect serum hepatitis E virus （HEV） antibody and liver HEV antigen in the slaughtered sheep from six counties of Xinjiang. The HEV infection rates were compared among the sheep from different regions, ages and breeds. The results showed that the serum HEV antibody positive rate was 14. 70/0 （38/258） in the slaughtered sheep, liver antigen HEV in- fection rate was 14. 0% （32/229）, and the infection rates between different regions were significantly different （P〈0. 05 ） ; for the serum or liver samples, the Rams infection rate （ 16. 7% and 15.0%） was higher than that of ewes （ 13.7% and 13.4%） ; for six breeds of sheep, the black sheep in Turpan area showed the highest infection rate, with the serum antibody rate of 17.1% and the liver HEV infection rate of 17.2%; There was significant difference in the HEV infection rate （P〈0. 01 ） between the one-year-old sheep and the sheep above two years old. The liver cells by transmission electron microscopy showed degeneration, characteristic vacuolation and other cytopathic effects. It suggests that HEV infection is prevalent in sheep in some areas of Xinjiang.
出处
《畜牧与兽医》
北大核心
2017年第5期126-130,共5页
Animal Husbandry & Veterinary Medicine
关键词
戊型肝炎
ELISA
免疫组化
超微病理
绵羊
新疆
hepatitis E
ELISA
immunohistochemistry
uhrastructural pathology
sheep
Xinjiang
