摘要
目的探讨卵巢癌细胞转移与组织、外周血中T淋巴细胞亚群失衡的相关性。方法选取89例行手术治疗的卵巢癌患者的手术标本，其中发生局部或远处转移40例（转移组），无远处或局部转移49例（非转移组）。采用流式细胞仪检测患者卵巢癌组织、癌旁组织和外周血CD4^＋和CD8^＋ T淋巴细胞，并采用酶联免疫吸附法（ELISA）检测外周血白细胞介素（IL）-6、IL-10、肿瘤坏死因子（TNF）-α和干扰素（IFN）-γ。结果转移组卵巢癌组织CD4^＋ T淋巴细胞明显高于非转移组（0.25 ± 0.04比0.20 ± 0.03），CD8^＋ T淋巴细胞明显低于非转移组（0.21 ± 0.03比0.24 ± 0.04），差异均有统计学意义（P〈0.01）。两组癌旁组织CD4^＋和CD8^＋ T淋巴细胞比较差异均无统计学意义（P 〉 0.05）。转移组外周血CD4^＋ T淋巴细胞明显低于非转移组（0.25 ± 0.04比0.28 ± 0.04），差异有统计学意义（P〈0.05）；两组外周血CD8^＋ T淋巴细胞比较差异无统计学意义（P〉0.05）。转移组外周血IL-6和TNF-α明显低于非转移组[（22.38 ± 5.58）ng/L比（25.82 ± 4.58）ng/L和（8.47 ± 2.35）ng/L比（10.74 ± 3.28）ng/L]，IL-10明显高于非转移组[（44.47 ± 12.26）ng/L比（39.48 ± 9.58）ng/L]，差异均有统计学意义（P〈0.01或〈0.05）；两组外周血IFN-γ比较差异无统计学意义（P〉0.05）。结论卵巢癌患者T淋巴细胞亚群失衡，主要表现为Th1/Th2细胞免疫向Th2细胞免疫漂移，与肿瘤的转移有关。
ObjectiveTo investigate the relationship between the imbalance of T cell subsets of the tissue and peripheral blood and ovarian cancer cell metastasis.MethodsThe surgical specimens of ovarian cancer of 89 patients who had underwent operation treatment were selected. Among them, local or distant metastasis was found in 40 cases （metastasis group）, and no local or distant metastasis was found in 49 cases （non-metastasis group）. The CD4^＋ and CD8^＋ T lymphocyte of ovarian cancer tissue, paraneoplastic tissue and peripheral blood were detected by flow cytometer, and the interleukin （IL）-6, IL-10, tumor necrosis factor （TNF）-α and interferon （IFN）-γ of peripheral blood were detected by enzyme linked immunosorbent assay （ELISA）.ResultsThe CD4^＋ T lymphocyte of ovarian cancer tissue in metastasis group was significantly higher than that in non-metastasis group： 0.25 ± 0.04 vs. 0.20 ± 0.03, the CD8^＋ T lymphocyte of ovarian cancer tissue was significantly lower than that in non-metastasis group： 0.21 ± 0.03 vs. 0.24 ± 0.04, and there was statistical difference （P〈0.01）. There were no statistical differences in CD4^＋ and CD8^＋ T lymphocyte of paraneoplastic tissue between 2 groups （P 〉0.05）. The CD4^＋ T lymphocyte of peripheral blood in metastasis group was significantly lower than that in non-metastasis group： 0.25 ± 0.04 vs. 0.28 ± 0.04, and there was statistical difference （P〈0.05）. There was no statistical difference in CD8^＋ T lymphocyte of peripheral blood between 2 groups （P〉0.05）. The IL-6 and TNF-α levels of peripheral blood in metastasis group were significantly lower than those in non-metastasis group： （22.38 ± 5.58） ng/L vs. （25.82 ± 4.58） ng/L and （8.47 ± 2.35） ng/L vs. （10.74 ± 3.28） ng/L, the IL-10 level was significantly higher than that in non-metastasis group： （44.47 ± 12.26） ng/L vs. （39.48 ± 9.58） ng/L, and there were statistical differences （P〈0.01 or〈0.05）. There was no statistical difference in IFN-γ of peripheral blood between 2 groups （P〉0.05）.ConclusionsThe imbalance of T cell subsets in patients with ovarian cancer is mainly manifested by the drift of Th1/Th2 cell immunity to Th2 cell, which is related to the metastasis of the tumor.
出处
《中国医师进修杂志》
2017年第5期390-394,共5页
Chinese Journal of Postgraduates of Medicine
关键词
卵巢肿瘤
T淋巴细胞亚群
肿瘤转移
免疫
细胞
回顾性研究
Ovarian neoplasms
T-lymphocyte subsets
Neoplasm metastasis
Immunity,cellular
Retrospective studies
