摘要
目的:观察肝癌组织中三结构域蛋白59(TRIM59)、Twist和E-钙黏蛋白(E-cadherin)的表达变化,并探讨其临床肿瘤学意义。方法:应用免疫组织化学方法检测80例肝癌组织及癌旁肝组织中TRIM59、Twist及E-cadherin的表达状况,分析其与临床病理参数的关系。结果:肝癌组织及癌旁肝组织中TRIM59的阳性率分别为76.3%,8.0%,差异具有统计学意义(P<0.05);肝癌组织及癌旁肝组织中Twist的阳性率分别为66.3%,6.0%,差异具有统计学意义(P<0.05);肝癌组织及癌旁肝组织中E-cadherin的阳性率分别为27.5%,90.0%,差异有统计学意义(P<0.05)。TRIM59、Twist及E-cadherin阳性表达均与肝癌病理学分级、分化、血管侵袭及TNM分期有关(P<0.05)。相关性分析表明,TRIM59与Twist表达呈正相关(P<0.05),TRIM59和E-cadherin表达呈负相关(P<0.05),Twist和E-cadherin表达也呈负相关(P<0.05)。生存分析结果表明,TRIM59是肝癌预后的独立因素。结论:联合检测TRIM59、Twist及E-cadherin有助于阐述肝癌发生发展、侵袭转移的分子机制,TRIM59有可能成为治疗肝癌的一个靶基因。
Objective To investigate the expressions of TRIM59, Twist and E-cadherin in hepatocellular carcinoma and their clinical significance. Methods The expressions of TRIM59, Twist and E-cadherin protein were tested by immunohistochemistry in 80 cases of hepatocellular carcinomas and the adjacent paracancerous tissues. Results The positive rate of TRIM59 in hepatocellular carcinoma was significantly higher than that in the adjacent paracancerous tissue （76.3% vs. 8.0%, P 〈 0.05）. Significant difference was also observed in the expression rate of Twist between the hepatocellular carcinomas and the paracancerous tissue （66.3% vs. 6.0%, P 〈 0.05）. The positive rate of E-cadherin in hepatocellular carcinoma was significantly lower than that in the adjacent paracancerous tissue （27.5% vs. 90.0%, P 〈 0.05 ）. The differences of the expression of TRIM59 in hepatocellular carcinoma of pathological grading, tumor differentiation, vascular invasion and clinical TNM stage were significant （P 〈 0.05）. The differences of the expression of Twist in hepatocellular carcinoma of pathological grading, differentiation, vascular invasion and clinical TNM stage was also significant （P 〈 0.05, respectively）. The differences of the expression of E-cadherin in hepatocellular carcinoma of pathological grading, differentiation, vascular invasion and clinical TNM stage were also significant （P 〈 0.05, respectively）. Significantly positive correlation was also found between TRIM59 and Twist by using spearman correlation analysis （P 〈 0.05 ）. Negative correlations were observed between TRIM59 and E-cadherin （P 〈 0.05）, and between Twist and E-cadherin （P 〈 0.05）. Survival analysis showed that TRIM59 expression was an independent prognostic factor in hepatocellular carcinoma. Conclusion TRIM59, Twist and E-cadherin protein expression might be associated with the development, invasion, and metastasis of hepatocellular carcinoma. TRIM59 may become a new target gene for the treatment of human hepatocellular carcinoma.
出处
《实用医学杂志》
CAS
北大核心
2017年第9期1453-1456,共4页
The Journal of Practical Medicine
基金
山东省医药卫生科技发展计划(编号:2016WS0002)
