摘要
蛹虫草和铁元素被证明对人体有着重要的生理作用。通过设计单因素和多因素正交试验,探讨蛹虫草富铁液体深层发酵条件的优化。试验证实,蛹虫草富铁液体深层发酵的最佳培养基配方为:大豆粉35 g/L、蔗糖30 g/L、KH_2PO_41.5 g/L、Mg SO_41.5 g/L、硫胺素5×10^(-5) g/L、Fe SO_4·7H2O 0.3 g/L。最佳培养条件为:pH6,温度26℃,发酵3 d,接种量5%,装液量100 mL/250 mL三角瓶。在此发酵条件下,测得富铁蛹虫草生物量为18.93 g/L,富集铁为16.01 mg/g。
As Cordyceps militaris and iron element have been proved to have important physiological functions to human body. Through designing the single factor and multi-factor orthogonal experiments to explore the opti-mization of submerged fermentation condition of iron-riched Cordyceps militaris. The best culture medium for-mula of submerged fermentation had been determined by experiments as follows：soybean meal 35 g/L, sugar 30 g/L, KH2PO41.5 g/L, MgSO41.5 g/L, thiamine 5×10-5 g/L, FeSO4·7H2O 0.3 g/L. The best culture condition had been achieved as follows：pH6, temperature 26℃, fermentation 3 days, inoculation quantity 5%, fluid volume 100 mL/250 mL of triangle flask. Under the optimal conditions of the submerged fermentation, it was measured that the biomass of iron-riched Cordyceps militaris was 18.93 g/L, and the enrichment of iron was 16.01 mg/g.
作者
李慧英
LI Hui-ying(Nantong Polytechnic College, Nantong 226002, Jiangsu, Chin)
出处
《食品研究与开发》
CAS
北大核心
2017年第7期153-159,共7页
Food Research and Development
关键词
蛹虫草
富铁
液体深层发酵
优化
Cordyceps militaris
iron-riched
submerged fermentation
optimization
