摘要
为了分析西方蜜蜂抗螨蜂种的群体遗传多态性情况,采用18对微卫星(SSR)引物对抗螨蜂种子一代和子二代,并与同场饲养不抗螨的本地意蜂和浙大蜜浆高产蜂种进行比对,共计22个群体进行遗传多样性检测.结果表明:遗传一致度在0.2时,抗螨蜂种子代发生一定的分化,其他两个品系基本一致.抗螨蜂种子代之间的亲缘关系较近(遗传距离在0.043 9以内),与本地意蜂和浙大蜜浆高产蜂种各自相互分开.从而对抗螨蜂种的选育和保护具有重要的指导意义.
Varroa-resistant honeybees of A . mellif era L . has a strong resistance to Varroa destructo , which were named as FI and F 2. Firstly the genetic diversity of F I and F 2 was investigated with 18 micro-satellite markers. Secondly, the mite-resistance bees of the local A . mellif era （Yunnan, Southwest Chi-na） and the high yield bees of royal jelly breed from Zhejiang University （ East China） were chosen for comparison analysis. The results show, in 22 sample colonies, when Nei＇s genetic identity index exceeded 0. 2, distinct genetic divergence was found filial generation of mite-resistance bees, while other breeds were the same nearly. The FI and F 2 of mite-resistance bees had a close descent relationship （the genetic dis-tances between them were 0. 0439） , with the local A . mellif era and the high yield bees of royal jelly breed from Zhejiang University separated from each other.
作者
张祖芸
杨若鹏
陈玲
余玉生
宋文菲
王艳辉
张学文
ZHANG Zu yun YANG Ruo-peng CHEN Ling YU Yu-sheng SONG Wen-fei WANG Yan-hui ZHANG Xue-wen(Sericultural and Apicultural Institute, Yunnan Academy of Agricultural Sciences, Mengzi Yunnan 661101, China College of Life Science and Technology, Honghe University, Mengzi Yunnan 661100 , China Research Institute of Biotechnology ＆ Genetic Germplasm,Yunnan Academy of Agricultural Sciences, Kunming 650223, China)
出处
《西南师范大学学报（自然科学版）》
CAS
北大核心
2017年第4期31-37,共7页
Journal of Southwest China Normal University(Natural Science Edition)
基金
国家蜂产业技术体系专项(CARS-45-SYZ 17)
关键词
西方蜜蜂
抗螨蜂种
遗传多样性
微卫星标记
A . mellif era L .
mite-resistance bees
genetic diversity
SSR makers
