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斑马鱼鳍和鳞片色素细胞的显微观察 被引量:9

Microscopical Observation on Pigment Cells in Fins and Scales of Zebrafish
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摘要 目的观察斑马鱼鳍和鳞片色素细胞的组成、分布及形态特征。方法应用光学显微镜对AB品系斑马鱼鳍和鳞片色素细胞的显微结构进行观察。结果斑马鱼鳍上分布有黑色素细胞和黄色素细胞,未观察到虹彩细胞。根据色素细胞的分布规律,将斑马鱼鳍分为有、无斑马鱼条纹两类。鳞片中分布有黑色素细胞、黄色素细胞和虹彩细胞。黑色素细胞较大,形态上主要分为呈树突状、颜色较浅、体积较大的I类黑色素细胞和呈团状、颜色较深、个体较小的Ⅱ类黑色素细胞。黄色素细胞个体最小,呈黄色或者橙黄色。虹彩细胞较长呈长棒状或梭形,数量最少。结论斑马鱼体表有黑色素细胞、黄色素细胞和虹彩细胞三种类型色素细胞,但鳍和鳞片在色素细胞组成和分布上存在着差异,鳞片中色素细胞的种类和形态特征较鳍中色素细胞更丰富。 Objective To observe the composition, distribution and morphological characteristics of pigment cells in the fins and scales of zebrafish. Method The microstructure of pigment cells in the fins and scales of AB strain of zebrafish was observed by optical microscope. Results All fins in zebrafish distributed melanophores and xanthophores, without iridophores. According to the distribution of the pigment cells, the zebrafish fins were divided into two types: with or without zebra stripes. There were melanophores, xanthophores and iridophores existed in scales. The melanophores were mainly divided into two types: type I was larger, obviously dendritic and light color; type II was smaller, nodular, dark color. Xanthophores were the smallest, yellow or orange yellow. Iridophores were a long rod like or fusiform, with smallest number. Conclusion There were three types of pigment cells in the surface of zebrafish including melanophores, xanthophores and iridophores. The composition and distribution of pigment cells in the fins and scales were different. The types and morphological characteristics of pigment cells in the scales were more abundant than that in fins.
作者 林金杏 冯丽萍 胡建华 高诚 LIN Jin-xing FENG Li-ping HU Jian-hua GAO Cheng(Shanghai Laboratory Animal Research Center, Shanghai 201203, China)
出处 《实验动物与比较医学》 CAS 2017年第2期94-101,共8页 Laboratory Animal and Comparative Medicine
基金 上海市科技发展基金实验动物研究项目(15140901000 16140904300)
关键词 斑马鱼鳍鳞片色素细胞 黑色素细胞 黄色素细胞 虹彩细胞 Zebrafish Fin Scale Pigment cell Melanophore Xanthophore Iridophore
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