摘要
本文研究了盐生杜氏藻（Ｄｕｎａｌｉｅｌａｓａｌｉｎａ，系中国科学院海洋研究所赠送）在２６℃、３０００ｌｘ试验条件下，不同氮、磷浓度对其细胞生长与β－胡萝卜素积累的作用．分别提供ＫＮＯ３或ＫＨ２ＰＯ４时，对盐藻生长的最适浓度分别为氮１ｍｍｏｌ／Ｌ，磷０．３ｍｍｏｌ／ｌ．而对细胞个体积累β－胡萝卜素的最适条件是无氮、无磷，经１０ｄ生长，其β－胡萝卜素含量分别达１１．２４ｐｇ／ｃｅｌ和１０．２８ｐｇ／ｃｅｌ．本文还讨论了以上结果的机理及意义，为建立盐藻高产β－胡萝卜素的控制模式提供了重要理论依据．
unaliella salina is a promissing resource ,an unicellular green alga, that accumulates large amounts of β-carotene when cultivated in special stress conditions.The cell line of D.salina was got from prof. Chen Jiaofen at Institute of Oceanography,Academic Sinica,Qingdao.The optimum parameters of N-KNO 3 and P-KH 2PO 4 for both algal important events, cell growth and accumulation of β-carotene ,were established basis on series of experiment with different concentration under the conditions at 36℃ and 3000lx. Results showed that the algal mximum growth rate appear at level of N and P as high as 1mmol/L and 0.3mmol/L respectively. Oppositely, the cellular maximum content of β-carotene was obtained at N-free of P-free, and each content of β-carotene as 11.24pg/cell of 10.82pg/cell successively in period of 10 days. Based on the above results, the controled mechanism on nitrogen and phosphate for available cell growth and accumulation of β-carotene was thus disscued.
基金
国家自然科学基金
浙江省自然科学基金
