摘要
目的探讨阿托伐他汀对高糖诱导人脐静脉内皮细胞（HUVECs）长链非编码RNAs（LncRNAs）分子转移相关肺腺癌转录物1（MALAT1）及炎症因子表达的影响。方法以实时定量PCR检测高糖（30mmol／L）刺激HUVECs不同时间后MALATl的表达水平；在高糖条件下，利用siRNA技术沉默细胞中MALATl的表达，或加阿托伐他汀处理，检测MALATl、白细胞介素（IL）-6和IL-8的表达水平。结果（1）高糖刺激HUVECs不同时间后，MALATl的表达出现了不同程度的升高（P〈0．05），在12h时MALATl表达增加最为显著（P〈0．01）．高糖刺激HUVECs12h后炎症因子1L-6和IL-8的表达和分泌水平也明显升高（P〈0．05）：（2）siRNA沉默MALATl表达可明显抑制高糖刺激的IL-6和IL-8表达和分泌（P〈0．05）；（3）阿托伐他汀明显抑制高糖刺激的MALATl、IL-6和IL-8表达（P〈0．05）。结论高糖可通过MALAT1介导内皮细胞炎症因子的分泌，而阿托伐他汀可明显抑制高糖刺激MALAT1的表达，降低炎症反应．
Objective To investigate the effect of atorvastatin on the expressions of long non-coding RNA （LncRNA） metastasis-associated lung adenocarcinoma transcript 1 （ MALAT1 ） and inflammation factors in human umbilical vein endothelial cells （HUVECs） stimulated by high glucose. Methods The expression of MALAT1 in HUVECs incubated with high glucose （30 mmol/L） for different time periods were detected by real-time PCR. Under high glucose condition, the expressions of MALAT1, interleukin-6 （ IL-6 ）, and interleukin-8 （ IL-8 ） in HUVECs were detected after MALAT1 was silenced by siRNA or atorvastatin was added. Results （ l ） After HUVECs were incubated with high glucose for different time periods, the expressions of MALAT1 were increased to some extent（P〈 0.05 ） , with the peak at 12h （ P〈0.01 ）. The levels of IL-6 and IL-8 expression and secretion were increased after HUVECs were stimulated by high glucose for 12h （ P〈0.05 ）. （ 2 ） The silence of MALAT1 markedly suppressed high glucose-stimulated expression and secretion of IL-6 and IL-8 （ P〈0.05 ）. （ 3 ） Atorvastatin significantly inhibited high glucose-stimulated expressions of MALAT1, IL-6, and IL-8 （ all P〈0.05 ）. Conclusions High glucose induces the secretion of inflammatory factors by stimulating MALAT1 expression in endothelial ceils. Atorvastatin significantly inhibits high glucose-stimulated MALAT1 expression and decreases inflammatory reaction.
出处
《中华内分泌代谢杂志》
CAS
CSCD
北大核心
2017年第4期330-334,共5页
Chinese Journal of Endocrinology and Metabolism
基金
国家自然科学基金项目（81260134.81560148）.广西硕士研究生创新项目（YCSZ2015218）
关键词
阿托伐他汀
高糖
转移相关肺腺癌转录物1
炎症反应
Atorvastatin
High glucose
Metastasis-associated lung adenocarcinoma transcript 1
Inflammatory reaction
