摘要
目的探讨不同浓度的阿托伐他汀和缬沙坦对高糖诱导人脐静脉内皮细胞损伤代谢记忆效应的保护作用。方法将人脐静脉内皮细胞分为正常对照组、甘露醇对照组、高糖组、阿托伐他汀低、中、高剂量组和缬沙坦低、中、高剂量组。其中高糖组、不同浓度（0．1、1和10μmol／L）阿托伐他汀及缬沙坦各组细胞于30mmol／L葡萄糖培养16h后，再以5．5mmol／L葡萄糖培养6d，应用酶联免疫吸附测定法检测各组细胞培养上清中血管细胞黏附分子1（VCAM-1）、单核细胞趋化蛋白1（MCP-1）和纤溶酶原激活物抑制剂1（PAI-1）水平。结果与正常对照组相比，高糖组内皮细胞上清中VCAM-1、MCP-1和PAI-1水平升高（均P〈0．05）。在糖浓度恢复正常后仍持续不降（均P〈0．05）。与高糖组相比，阿托伐他汀和缬沙坦预处理可以明显降低VCAM-1、MCP-1和PAI-1水平（均P〈0．05）。结论阿托伐他汀和缬沙坦可通过降低VCAM-1、MCP-1和PAI-1水平，对高糖诱导内皮细胞损伤“代谢记忆”效应产生保护作用。
Objective To explore the effects of atorvastatin and valsartan on high glucose-induced human umbilical vein endothelial cells （HUVECs） injury. Methods Cultured HUVECs were divided and assigned to 9 groups： normal control group, mannitol control group, high glucose group, low dose atorvastatin group （0. 1 μmol/L） , medium dose atorvastatin group （1 μmol/L） , high dose atorvastatin group （10 μmol/L） , low dose valsartan group （0. 1 μmol/L）, medium dose valsartan group （ 1μmol/L）, and high dose valsartan group （ 10 μmo]./L）. HUVECs were pretreated with or without 30 mmol/L glucose plus various concentrations of atorvastatin and valsartan （0.1, 1, 10 μmol/L） for 16 hours and then incubated with 5.5 mmol/L glucose for 6 days. The levels of vascular cell adhesion molecule 1 （ VCAM-1 ）, monocyte chemotaetic protein 1 （ MCP-1 ）, and plasminogen activator inhibitor 1 （PAI-1） in the culture supernatant were measured by enzyme-linked immunosorbent assay. Results Compared with normal glucose group, hyperglycemia memory increased the levels of VCAM-1, MCP-1, and PAI-1 （ all P〈0.05 ） , which were still maintained at high levels even after withdrawal of high glucose. Atorvastatin and valsartan treatment decreased the levels of VCAM-1, MCP-1, and PAl-1 （ all P〈0.05 ）. Conclusion Atorvastatin and valsartan may lower the secretion of VCAM-1, MCP-1, and PAI-1, and prevent high glucose memory-induced injury to endothelial cell.
出处
《中华内分泌代谢杂志》
CAS
CSCD
北大核心
2015年第12期1055-1059,共5页
Chinese Journal of Endocrinology and Metabolism
基金
2013年中华医学会基金项目（13020220407）
关键词
阿托伐他汀
缬沙坦
人脐静脉内皮细胞
代谢记忆
Atorvastatin
Valsartan
Human umbilical vein endothelial cell
Metabolic memory
