摘要
目的研究凹叶厚朴适用于细胞悬浮培养的愈伤组织。方法通过对基本培养基(B5、MS和1/2MS)、外植体(茎段、顶芽和叶片)、激素浓度及组合进行筛选的方法进行厚朴愈伤组织诱导和继代研究。结果 B5为最适合厚朴愈伤组织诱导的基本培养基;顶芽是最佳诱导愈伤组织的材料;最适合厚朴愈伤组织诱导的培养基为B_5+2.0 mg/L 2,4-D+1.0 mg/L 6-BA;最佳继代培养基为B_5+2.0 mg/L NAA+1.0 mg/L 6-BA,愈伤组织生长倍数达到2.87倍。结论初步筛选出凹叶厚朴用于细胞悬浮培养的愈伤组织,为凹叶厚朴细胞培养奠定基础。
Objective To study the callus of Magnolia officinalis suitable for cell suspension culture. Methods Callus induction and subculture study were carried out through the selection of basic mediums （B5, MS and 1/2MS）, explants （stems, phyllophores and leaves） and concentrations of hormone. Results The best basic medium for callus induction of Magnolia biloba was B5. Phyllophor is the best explant for callus induction of magnolia biloba. The best medium for callus induction of magnolia biloba was B5＋2.0 mg/L 2,4-D＋1.0 mg/L 6-BA. The best subculture medium for callus of Magnolia officinalis was B5＋2.0 mg/L NAA＋1.0 mg/L 6-BA, callus growth rate reached 2.87 times. Conclusion The callus of Magnolia officinalis suit-able for cell suspension culture has been preliminary screened, which laying the foundation for cell suspension culture of Magnolia officinalis.
出处
《湖南中医药大学学报》
CAS
2017年第4期365-368,共4页
Journal of Hunan University of Chinese Medicine
基金
"十二五"国家科技支撑计划项目(2011BAI01B06)资助
福建省科技创新平台(2008Y2001)资助
关键词
凹叶厚朴
愈伤组织
悬浮培养
Magnolia officinalis
callus
suspension culture
