摘要
目的探讨线粒体分裂相关蛋白(Drp-1)与糖尿病周围神经病变(DPN)的关系及木丹颗粒的干预作用。方法 50只大鼠随机分为糖尿病对照(DM)组、木丹颗粒(MD)组、α-硫辛酸(LA)组和木丹颗粒联合α-硫辛酸(CT)组,另设正常(NC)组。12周后检测各组坐骨神经传导速度(sNCV)、血清中总超氧化物歧化酶(T-SOD)、谷胱甘肽(GSH)、丙二醛(MDA)和过氧化氢酶(CAT)的水平及坐骨神经中Drp-1、Bax、Bcl-2mRNA相对表达量。结果 NC、DM、MD、LA、CT组Drp-1 mRNA相对表达量分别为(0.99±0.07)、(1.78±0.08)、(1.48±0.09)、(1.44±0.10)和(1.30±0.05);Bax mRNA相对表达量分别为(1.09±0.21)、(7.08±0.86)、(4.95±0.83)、(4.52±0.88)和(2.86±0.54);Bcl-2 mRNA相对表达量分别为(7.80±0.40)、(2.99±0.64)、(4.06±0.81)、(4.09±0.80)和(5.62±0.83)。与NC组比较,DM组T-SOD、GSH、CAT和sNCV降低,MDA升高(P<0.05);与DM组比较,MD、LA及CT组TSOD、GSH、CAT和sNCV升高,MDA降低(P<0.05),其中CT组变化更明显。结论 Drp-1表达升高可能参与DPN发病,木丹颗粒能干预Drp-1表达,减少细胞凋亡,并降低氧化应激水平,对糖尿病大鼠坐骨神经起到一定的保护作用。
Objective To explore the relationship between Dynamin-related protein-l（Drp-1） and diabetic peripheral neuropathy, and effects of Mudan particles. Methods A total of 50 rats were randomly divided into four groups：diabetes mellitus group（DM）, Mudan particles group（M D）, a-lipoic acid groups （LA）, combined treatment group （CT）. Healthy rats were selected as normal control （NC） group. After follow up for 12 weeks, the sciatic nerves conduction velocity（sNCV）was measured . Serum total- Superoxide dismutase（T-SOD）, glutathione（GSH）, maglondialdehyde（MDA）, and Catalase（CAT） levels were tested. The expression of Drp-1 ,Bax and Bcl-1 mRNA in sciatic nerves were measured. Results The expressions of DrrrlmRNA were（0. 99±0. 07）, （1. 78±0. 08）, （1. 48±0.09）, （1. 44±0. 10）, （1.30 ±0. 05） ;the expressions of Bax mRNA were（1.09±0.21）, （7. 08±0. 86）, （4. 95±0. 83）, （4. 52±0.88）, （2. 86±0. 54）, and the expressions of Bcl-2 mRNA were（7.80±0. 40）, （2.99±0. 64）, （4. 06 ±0. 81）, （4. 09±0. 80）,（5. 62±0. 83） in NC,DM,MD, LA and CT group respectively. The level of T-SOD,GSH and CAT and sNCV were lower,MDA were higher in DM group than in NC group （P〈0. 05）. The level of T-SOD,GSH,CAT a and sNCV were higher, MDA were lower（P〈0. 05） in MD, LA and CT group, especially in CT group than in DM group. Conclusion Drp-1 over-expression may participate in the pathogenesis of DPN. Mudan granule can interfere with Drp-1 mRNA expression and reduce apoptosis which shows a certain protective effect on sciatic nerve of diabetic rats.
作者
陈慧晓
杨俊朋
梁萌萌
张园园
赵志刚
CHEN Hui- xiao YANG Jun-peng LIANG Meng-meng et al(Department of Endocrinology and Metabolism, The People ＇ s Hospital of Zhengzhou University, Zhengzhou 450003, Chin)
出处
《中国糖尿病杂志》
CAS
CSCD
北大核心
2017年第4期359-362,共4页
Chinese Journal of Diabetes
基金
郑州市科技计划项目(131PLJRC667)
关键词
木丹颗粒
糖尿病神经病变
发动相关蛋白-1
氧化应激
线粒体功能障碍
.
Mudan particles
Diabetic neuropathy~ Dynamin-related protein-1 （Drp-1）
Oxidativestress（OS）
Mitochondrial dysfunction
