摘要
目的:比较不同方法制作胸腹水细胞块的效果以优化胸腹水细胞块制作程序,并对其临床价值进行探讨。方法:以2014年3月至2015年3月我科收集的胸腹水标本120例为研究对象,将每样标本平均分成三组,使用三种不同方法(试管包埋法、直接离心法、细胞块试剂盒法)制作胸腹水细胞块。对不同方法制作细胞块的成功率、完整性及细胞切片恶性细胞的检出率进行考察与比较。结果:细胞块试剂盒法成功率最高,为96.67%,试管包埋法次之,成功率为92.50%,二者相比无显著差异(P>0.05)。直接离心法制作成功率为80.83%,远远低于试管包埋法及细胞块试剂盒法,差异有统计学意义(P<0.05)。细胞块试剂盒法制作的细胞块完整性最高,完整标本所占比例为96.67%,试管包埋法次之,完整率为94.17%,二者相比无显著差异(P>0.05)。直接离心法制作完整率为68.33%,远远低于试管包埋法及细胞块试剂盒法,差异有统计学意义(P<0.05)。三种方法恶性细胞检出率以细胞块试剂盒最高,与其他两种方法比较差异有统计学意义(P<0.05)。结论:细胞块试剂盒法制作胸腹水细胞块具有最高的成功率及完整性,并且可以显著提高恶性细胞检出率,值得广泛使用。
Objective： To compare the efficacy of different methods in preparation of pleural and peritoneal effusion cell blocks in order to optimize the procedure, and explore the clinical efficacy. Methods： 120 cases pleural and peritoneal effusion selected in our de- partment from March 2014 to March 2015 were divided into 3 groups using different methods （tube embedding method, centrifugal method and cell blocks kit method） in preparation ofceU blocks. The success rate, completeness and detection rate of malignant cells of 3 groups were observed and compared. Results： The success rate of cell blocks kit （96.67 %） was the highest, then the tube embedding method （92.50 %）, which had no statistically difference （P〉0.05）. The success rate of direct centrifugal method was obviously lower than that of the other two methods with statistically significance （P〈0.05）. The completeness of cell blocks kit was the highest with the rate of 96.67 %, then the tube embedding method （94.17 %）, which had no statistically difference （P〉0.05）. The completeness rate of direct cen- trifugal method was 68.33 %, which was obviously lower than that of the others with statistically difference （P〈0.05）. The detection rate of malignant cells of cell blocks kit was higher than that of the others with statistically significance （P〈0.05）. Conclusions： Using cell blocks kit in preparation of pleural and peritoneal effusion can receive the best success and completeness rate, and contribute to improve the detection rate of malignant cells, worthy of application.
出处
《现代生物医学进展》
CAS
2017年第7期1357-1359,1346,共4页
Progress in Modern Biomedicine
关键词
胸腔积液
腹水
细胞块
临床价值
Pleural effusion
Peritoneal effusion
Cell blocks
Clinical value
