摘要
目的探讨液基细胞学(TCT)与免疫细胞化学技术相结合在疑难胸腔积液鉴别诊断中的临床价值。方法应用液基细胞学技术,对临床初次送检的胸腔积液进行诊断,对可疑癌细胞且与反应性间皮细胞不易鉴别,临床再次送检的30例胸腔积液制备石蜡细胞块,并做免疫细胞化学,标记抗体Ber-EP4、B72.3、MOC-31、Calretin in(简称CR)。结果 30例胸腔积液中腺癌细胞26例,非典型间皮细胞4例,腺癌细胞中表达Ber-EP4 23例(占88.5%),B72.3 20例(占76.9%),MOC-31 18例(占69.2%),CR 1例(占3.8%)。非典型间皮细胞仅表达CR(占100%)。结论液基细胞学与免疫细胞化学技术相结合在疑难胸腔积液鉴别诊断中有很重要的临床意义,Ber-EP4、B72.3、MOC-31和CR抗体的联合应用可用于鉴别诊断腺癌细胞及间皮细胞,值得推广应用。
Objective To investigate the application value of ThinPrep Cytological Test（TCT） combined with Immunocytochemistry technology in diagnosis of complicate pleural effusion.Methods Analyzing the all of first-time examinational pleural effusion were detected with TCT,thereinto,30 samples of second-time examination and difficulty to differentiate diagnosis were made into Cell Blocks and marked with Ber-EP4,B72.3,MOC-31,Calretinin（CR）Results Of the 30 cases detected by immunocytochemistry,26 cases were diagnosed as Glandular cancer and 4 cases were Atypical Mesotheslial Cell.Of the 26 glandular cancer cases,23（88.5%） cases express Ber-EP4,20（76.9%） express B72.3,18（69.2%） express MOC-31 and only 1（3.8%） express CR.But all of Atypical Mesotheslial Cell（100%） only express CR.Conclusion TCT combined with Immunocytochemistry had important clinical signification in complicated pleural effusion diagnosis and differential diagnosis.A combination of Ber-EP4,B72.3,MOC-31 with CR should be popularized in differentiate between glandular cancer cell and atypical Mesotheslial Cell.
出处
《中华全科医学》
2011年第10期1556-1557,F0003,共3页
Chinese Journal of General Practice
