摘要
采用化学交联法,以1-(3-二甲氨基丙基)-3-乙基碳二亚胺(EDC)为脱水剂、4-二甲基氨基吡啶(DMAP)为催化剂,将透明质酸(Hyaluronic acid,HA)接枝到普鲁兰(Pullulan,Pu)糖长链上,制备透明质酸-普鲁兰糖(HA-Pu)新型材料.傅里叶变换红外和氢核磁表征显示,成功合成HA-Pu材料,并用~1H NMR法确定HA-Pu的透明质酸取代度.合成新材料冻干后可压制获得HA-Pu膜,扫描电镜下观察,HA-Pu膜为层状结构,具备很多微小孔洞.体外酶降解实验表明,新型HA-Pu膜较透明质酸具有更好的抵抗酶降解性能.HA-Pu新材料合成有望拓展透明质酸在医药领域的应用.
A series of hyaluronic acid grafted pullulan（HA-Pu）were prepared by esterification reaction, using 1-（3-Dimethylaminopropyl）-3-ethylcarbodiimide （EDC） as dehydrating agent and 4-dimethylaminopyridine（DMAP）as catalyst.The structure of HA-Pu was confirmed by the Fourier transform infrared spectrum（FTIR）and proton nuclear magnetic resonance（^1H NMR）, and the degree of substitution（DS） of hyaluronic acid moiety was determined by 1H NMR.The freeze-dried HA-Pu materials could be compressed to obtain a HA-Pu film. The HA-Pu film was observed by scanning electron mieroscopy（SEM）, which obtained the layered microstructure with a lot of small holes. Compared with hyaluronic acid, the HA-Pu materials have better ability to resist enzyme degradation in vitro. Therefore, HA-Pu should be a promising novel material to expand the application of hyaluronic acid in pharmaceatics.
出处
《河北大学学报（自然科学版）》
CAS
北大核心
2017年第2期141-146,共6页
Journal of Hebei University(Natural Science Edition)
基金
河北省高等学校科学技术研究项目(ZD2016102)
河北大学研究生创新项目(X2016071)
关键词
透明质酸
普鲁兰糖
化学接枝
体外酶降解
hyaluronic acid
pullulan
chemical grafting
enzyme degradation in vitro
