摘要
目的:考察水飞蓟宾葡甲胺盐对肝纤维化大鼠的疗效和可能机制。方法:将肝纤维化大鼠相随机分为4个组别,即模型组、水飞蓟宾葡甲胺盐高剂量组120 mg/kg、水飞蓟宾葡甲胺盐中剂量组60 mg/kg和水飞蓟宾葡甲胺盐低剂量组30 mg/kg,将同期正常大鼠作为正常对照组,每组10只,连续给药4周。结果:试验全程无死亡大鼠。与正常组比,模型组大鼠体重减少,少动,毛发暗淡,肝脏指数减少(P<0.05),脾脏指数增加(P<0.05),与模型组相比,水飞蓟宾葡甲胺各组体重明显增加(P<0.05),活动较好,毛发色泽有改善,肝脏指数明显增加(P<0.05),脾脏指数明显降低(P<0.05)。与对照组比,模型组的ALT、AST和TBIL增高(P<0.05),ALB降低(P<0.05),与模型组相比,水飞蓟宾葡甲胺各组的ALT、AST和TBIL显著降低(P<0.05),ALB显著增高(P<0.05)。与对照组比,模型组的TG、TC和LDLC增高(P<0.05),与模型组相比,水飞蓟宾葡甲胺各组的TG、TC和LDLC显著降低(P<0.05)。与对照组相比,模型组的LXRα及SREBP1c蛋白显著增高(P>0.05),与模型组相比,水飞蓟宾葡甲胺各组的LXRα及SREBP1c蛋白显著降低(P<0.05)。模型组大鼠的肝小叶消失,中度增生的显微组织将肝小叶分为不同的假小叶,大部分肝细胞明显脂肪变性,点灶状坏死,水飞蓟宾葡甲胺各组的肝组织,肝小叶恢复,肝细胞脂肪变性改善,坏死点灶明显减少。结论:水飞蓟宾葡甲胺盐具有治疗肝纤维化的作用,在改善肝功能和降低血脂方面具有显著优势,与降低肝组织的LXRα和SREBP1c表达有关,但降低上述两种蛋白的机理仍有待进一步考察。
Objective： To investigate the efficacy of Silybinin meglumine on hepatic fibrosis rats and possible mechanisms. Methods： The liver fibrosis rats were randomly divided into 4 groups,the model group,Silybinin meglumine 120 mg / kg group,Silybinin meglumine dose group 60 mg / kg and Silybinin meglumine low dose group 30 mg / kg,and the control group. All groups had been treated for 4 groups. Results： No deaths rat. Compared with the control group,the reduced body weight,less dynamic,dark hair,decreased liver and spleen indexes,increased ALT,AST,TBIL,TG,TC and LDLC,and the decreased ALB,and the increased LXRαand SREBP1 c had been observed in the model group（ P〈0. 05）. Compared with the model group,better activity and body weight,the increased liver and spleen indexs decreased ALT,AST,TBIL,TG,TC and LDLC,and the increased ALB,and the decreased LXRα and SREBP1 c had been observed in the Silybinin meglumine groups（ P 〈 0. 05）,in a way of dose-depended. Conclusion： The Silibinin meglumine can treat liver fibrosis,by improving liver function,lowing lipid and decreaseing LXRα and SREBP1 c expression in liver tissue. But the mechanism of two proteins reduced remains for further investigation.
作者
陈应康
佘福强
刘大腾
朱建超
刘燕
田培燕
CHEN Ying-Kang SHE Fu-Qiang LIU Da-Teng ZHU Jian-Chao LIU Yan TIAN Pei-Yan(Qiannan Medical College for Nationalities ,Nanzhou 558000, Chin)
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2017年第3期360-364,共5页
Chinese Journal of Immunology
基金
黔南民族医学高等专科学校科研基金项目(No.QNYZ 2013字2号)
关键词
水飞蓟宾葡甲胺盐
肝纤维化
肝功能
血脂
肝组织蛋白
Silibinin meglumine
Liver fibrosis
Hepatic function
Lipids
Liver tissue protein
