摘要
目的观察麻黄碱对IL-17诱导的人永生化角质形成细胞HaCaT分泌CCL20的影响。方法将HaCaT细胞随机分为6组。A组加不含药物的培养液,B组加含20 ng/m L IL-17的培养液,C、D、E分别加含20 ng/m L IL-17联合800、1 200、1 600μg/m L麻黄碱的培养液,F组加含20 ng/m L IL-17联合50μg/m L MTX的培养液。培养36h后,采用ELISA法测定细胞上清液CCL20,采用RT-PCR法测定细胞中的CCL20 mRNA。结果 A、B、C、D、E、F组HaCaT细胞上清液CCL20水平分别为(97.72±1.86)、(159.43±7.09)、(147.05±2.25)、(138.60±8.04)、(126.66±7.04)、(114.39±3.90)pg/m L,HaCaT细胞CCL20 mRNA相对表达量分别为0.91±0.08、7.22±0.32、4.95±0.33、2.79±0.19、1.79±0.49、1.80±0.47;B、C、D、E、F组均高于A组(P均<0.05),D、E、F组均低于B组(P均<0.05),C、D组均低于F组(P均<0.05)。结论麻黄碱能有效抑制IL-17诱导的HaCaT细胞分泌CCL20,为麻黄治疗银屑病提供一定的理论依据。
Objective To observe the effect of ephedrine on CCL20 secretion of IL-17-induced immortalized human keratinocytes（ HaCaT）. Methods HaCaT cells were randomly divided into 6 groups. Cells in the group A were only treated by DMEM. Cells in the group B were treated with 20 ng/m L IL-17 nutrient solution. Cells in the groups C,D and E were treated with 800,1 200 and 1 600 μg/m L ephedrine medium including 20 ng/m L IL-17. Cells in the group F were treated with 20 ng/m L IL-17 combined with 50 μg/m L MTX culture medium. After 36 h,ELISA was performed to determine the expression of CCL20 protein. RT-PCR was used to assess the expression of CCL20 mRNA. Results The expression levels of CCL20 protein in the cultural supernatant of HaCaT cells of groups A,B,C,D,E and F were（ 97. 72 ±1. 86）,（ 159. 43 ± 7. 09）,（ 147. 05 ± 2. 25）,（ 138. 60 ± 8. 04）,（ 126. 66 ± 7. 04）,（ 114. 39 ± 3. 90） pg/m L,respectively. The relative CCL20 mRNA expression in HaCaT cells of groups A,B,C,D,E,F were 0. 91 ± 0. 08,7. 22 ±0. 32,4. 95 ± 0. 33,2. 79 ± 0. 19,1. 79 ± 0. 49,1. 80 ± 0. 47,respectively. Groups B,C,D,E and F were higher than group A（ all P 0. 05）; groups D,E,F were lower than group B（ all P 0. 05）; groups C and D were lower than group F（ all P 0. 05）. Conclusion Ephedrine can inhibit the CCL20 secretion of IL-17-induced HaCaT cells,and it provides a theoretical basis for the treatment of psoriasis by ephedrine or even diaphoresis.
作者
蒋燕
郭顺
邹悦
陈力
JIANG Yan GUO Shun ZOU Yue CHEN Li(First Clinical Medical College of Nanjing University of Chinese Medicine, Nanjing 210023, China)
出处
《山东医药》
CAS
北大核心
2017年第10期24-27,共4页
Shandong Medical Journal
基金
国家自然科学基金资助项目(81603626)
江苏省中医院院级课题(Y14020)
