摘要
目的构建大鼠DN模型,探讨外源性予腺病毒(Ang-1)对肾脏的血管生成标志物血栓调节蛋白-1(TM-1)和Ki-67的影响及肾新生血管生成方面的作用。方法将糖尿病大鼠分为Ang-1腺病毒组、空白载体组和DM模型组。Ang-1腺病毒组造模完成后8周,单次注射Ang-1腺病毒,剂量0.05ml/只;空白载体组单次注射Ang-1腺病毒的载体;DM模型组单次注射同剂量的0.9%生理盐水。另有正常对照组(NC),单次注射同剂量的0.9%生理盐水。注射后8、12、20和28周,分别对各组24h尿蛋白定量、肾组织的TM-1和Ki-67进行检测。结果 Ang-1腺病毒组、空白载体组和DM模型组24h尿蛋白表达较NC组升高(P<0.05),肾小球组织中TM-1和Ki-67表达较NC组增强(P<0.05),20周达峰值,各时点Ang-1腺病毒组的TM-1和Ki-67均强于同时点空白载体组和DM模型组(P<0.05)。24h尿蛋白与糖尿病大鼠肾组织的TM-1和Ki-67表达均呈正相关(P<0.05)。结论糖尿病大鼠的肾小球组织有新生血管生成,Ang-1具有促进糖尿病大鼠的肾小球组织有新生血管生成作用,TM-1和Ki-67可能参与其中。
Objective To explore the effect of Angiopoietin-1（Ang-1）on thrombomodulin-1（TM-1）and Ki-67 expression in kidney of diabetes mellitus（DM）rats and the effect of Ang-1 on kidney microvascular structure and function. Methods DM rats were divided into 3groups：Ang-1group,blank carrier group and DM model group.Health rats were selectd as the control group（NC）.Ang-1group,blank carrier group and DM model group were injected with Adenovirus Ang-1,Adenovirus blank carrier and natural salts solution,respectively.Data of 24 hours urinary protein,TM-1 and Ki-67 expression in kidney after 8,12,20,28 weeks were collected and compared. Results Compared with NC group,24 hours urinary protein increased in the three DM groups（P〈0.05）.Compared with NC group,the expressions of TM-1and Ki-67 in glomerulus increased in the three DM groups（P〈0.05）and reached peak in 20 weeks.Compared with blank carrier group and DM model groups,the expressions of TM-1and Ki-67 increased in Ang-1group（P〈0.05）.24 hours urinary protein had positive relationship with TM-1and Ki-67 in kidney in DM rats. Conclusion Ang-1may promote angiogenesis of glomerul tissue in DM rats by increasing the expression of TM-1and Ki-67.
作者
张子阳
曹明燕
黄妍
杨亦彬
ZHANG Zi-yang CAO Ming- yan HUANG Yan et al.(Department of Nephropathy Rheurnatology, Zunyi Medical College Affiliated Hospital, 563003 Zunyi , Chin)
出处
《中国糖尿病杂志》
CAS
CSCD
北大核心
2017年第3期255-258,共4页
Chinese Journal of Diabetes
基金
国家自然科学基金(81260118)
贵州省优秀科技教育人才省长专项资金[黔省专合字(2005-242)]
合肥医学院博士科研启动资金(2006-13)
