摘要
目的观察T2DM患者外周血microRNA-155(miR-155)、细胞核因子-κB(NF-κB)和可溶性细胞间黏附分子-1(sICAM-1)的表达情况,探讨miR-155在T2DM合并血管病变中的作用。方法选取T2DM患者165例和健康体检者(NC)60名,分为无血管病变组、有血管病变组,有血管病变组进一步分为微血管病变组、大血管病变组、微血管病变+大血管病变组;依据24hUAlb水平分为尿白蛋白正常(NAU)组、微量白蛋白尿(MAU)组、大量白蛋白尿(MAAU)组。RT-PCR检测外周血miR-155、NF-κB和sICAM-1的表达变化。结果与NC组比较,T2DM组miR-155、NF-κB、sICAM-1表达升高[(1.00±0.12)vs(1.82±0.71),(1.04±0.33)vs(2.28±0.66),(1.03±0.30)vs(1.88±0.80)](P<0.05);与无血管病变组比较,有血管病变组miR-155、NF-κB、sICAM-1表达升高[(1.34±0.29)vs(1.95±0.73),(1.65±0.16)vs(2.40±0.65),(1.07±0.41)vs(2.01±0.77)](P<0.05);微血管病变+大血管病变组miR-155表达高于大血管病变组[(2.36±0.61)vs(1.77±0.59)](P<0.05),NF-κB也高于微血管病变组和大血管病变组(P<0.05);与NAU组比较,MAU组和MAAU组miR-155表达升高,且MAAU组高于MAU组[(1.04±0.20)vs(1.41±0.49)vs(2.64±0.52)](P<0.05),NF-κB、sICAM-1也高于NAU组(P<0.05)。Pearson相关分析显示,miR-155与NF-κB、sICAM-1呈正相关(t=4.235、9.728,P<0.01)。结论 T2DM患者及不同血管并发症组miR-155表达升高,且与NF-κB、sICAM-1升高趋势一致,提示miR-155的炎症调控机制可能参与糖尿病慢性血管病变的发生发展。
Objective To investigate the expression of miR-155,nuclear factor kappa B（NF-κB）and soluble intercellular adhesion molecule-1（sICAM-1）in peripheral blood in patients with type 2diabetes mellitus（T2DM）,and to explore the role of miR-155 in vascular lesions of T2 DM. Methods A total of 165T2 DM patients and 60 health subjects from health examinations were enrolled in this study.All the subjects were divided into two groups：subjects without vascular lesions group and vascular disease group.Vascular disease group was further divided into microangiopathy group,macroangiopathy group,microangiopathy＋ macroangiopathy group;based on 24 hUAlb level normal urinary albumin（NAU）group,microalbuminuria（MAU）group,macroalbuminuria（MAAU）group.MiR-155,NF-κB and sICAM-1in peripheral blood were tested by RT-PCR.Single factor analysis of variance was used for comparison among groups.Stepwise regression analysis was used for correlation factors analysis. Results The expression of miR-155,NF-κB and sICAM-1were significantly higher in T2 DM group than in NC group[（1.82±0.71）vs（1.00±0.12）,（2.28±0.66）vs（1.04±0.33）,（1.88±0.80）vs（1.03±0.30）,P〈0.05].The level of miR-155,NF-κB,sICAM-1were significantly higher in T2 DM vascular lesions group than in subjects without vascular lesions group[（1.95±0.73）vs（1.34±0.29）,（2.40±0.65）vs（1.65±0.16）,（2.01±0.77）vs（1.07±0.41）,P 〈0.05].The expression of miR-155 was higher in microangiopathy＋ macroangiopathy group than in macroangiopathy group [（2.36±0.61）vs（1.77±0.59）,P 〈 0.05].The expression of NF-κB was also significantly higher in microangiopathy ＋macroangiopathy group than in microvascular disease group and macroangiopathy group（P〈0.05）.The level of miR-155 was significantly higher in group MAU group and MAAU group than in NAU group[（1.41±0.49）,（2.64±0.52）vs（1.04±0.20）,P〈0.05],and NF-κB and sICAM-1were also higher than NAU group（P〈0.05）.Multiple stepwise regression analysis showed that miR-155 was positively correlated with NF-κB and sICAM-1（t=4.235,9.728,P〈0.01）. Conclusion The expression of miR-155 increases in T2 DM patients with vascular complications,and this trend is the same as NF-κB and sICAM-1.It suggests that miR-155 maybe involves in the pathogenesis of diabetic chronic vascular disease.
作者
郭丽婷
高志红
葛焕琦
GUO Li-ting GAO Zhi-hong GE Huan-qi(Department of Endocrinology, Teda International Cardiovascular Hospital, Tianjin 300457 ,Chin)
出处
《中国糖尿病杂志》
CAS
CSCD
北大核心
2017年第3期213-217,共5页
Chinese Journal of Diabetes
基金
天津市滨海新区卫生计生委科技项目(2016BWΚY025)
