摘要
目的探讨SD大鼠急性心肌梗死(AMI)与血清卵泡抑素样蛋白1(FSTL1)及其相关miR-200b-3p的表达情况。方法 20只SD大鼠分为AMI组和Sham组,AMI组结扎大鼠左冠状动脉前降支,而Sham组不结扎,术后1d收集两组大鼠静脉血和心脏组织,采用ELISA法检测大鼠血浆cTnI和FSTL1的表达,用qRT-PCR法检测大鼠血浆、组织rno-miR-200b-3p和组织FSTL1的表达,采用Western blot法检测组织FSTL1的表达。结果 AMI组大鼠心肌梗死后1d血浆FSTL1的表达较Sham组明显升高(P<0.05),其ROC曲线AUC为1.000(P<0.05);而AMI组大鼠心肌梗死后1d心脏组织中FSTL1的表达无论在基因水平还是蛋白水平都较Sham组明显降低(P<0.05);AMI组大鼠术后1d血浆中rno-miR-200b-3p的表达较Sham组明显降低(P<0.05),而AMI组大鼠术后1d心脏组织中rno-miR-200b-3p较Sham组明显升高(P<0.05)。结论 FSTL1和rno-miR-200b-3p都可能与大鼠AMI有关,FSTL1可以作为AMI的心肌标志物,rno-miR-200b-3p可能通过调控FSTL1的表达而参与AMI发病机制的炎症反应、氧化应激和细胞凋亡等过程。
Objective To study the mechanism and the relationship of the follistatin-like protein 1 and miRNA-200 in acute myocardial infarction （AMI） in rats. Methods 20 SD rats were divided into AMI group and Sham group. AMI group was operated with the left anterior descending coronary artery ligation, and Sham group was not ligated. Venous blood and heart tissue were collected postoperative ld, plasma cTnI and FSTL1 expression were tested by ELISA. The plasma and heart rno-miR-200b-3p were detected by qRT-PC, and FSTL1 protein levels were performed by Western blot. Results The plasma FSTL1 expression of AMI group were increased significantly compared with that of Sham group （P〈0.05）. The AUC was 1. 000 （P〈0.05）. mRNA level and protein level of FSTL1 of AMI group were significantly lower in that of Sham group （P〈0.05） at postoperative 1d. The plasma rno-miR-200b-3p expression in AMI group was significantly decreased compared with that in Sham group （P〈0.05）. The heart tissue rno-miR-200b-3p expression in AMI rats were significantly increased compared with that in Sham group （P〈0.05）. Conclusion FSTL1 and rno-miR 200b-3p may be associated with AMI, and FSTL1 can be used as a biomarker of myocardial injury, and rno-miR-200b-3p may involve in inflammation, oxidative stress and cell apoptosis in AMI by mediated FSTL1.
出处
《西部医学》
2017年第3期316-321,共6页
Medical Journal of West China
基金
国家自然科学基金(81270262)
