摘要
【目的】了解2012–2015年江淮地区猪丹毒杆菌分离株血清型分布、spa A基因遗传进化关系和基因分型特征。【方法】收集临床分离鉴定的42株猪丹毒杆菌,应用琼脂扩散沉淀实验、PCR扩增和序列分析技术、脉冲场凝胶电泳分型技术(PFGE)分别测定分离株的血清型、spa A基因遗传变异性及PFGE基因型。【结果】42株猪丹毒杆菌分离株血清型均为1a型;spa A基因与猪丹毒杆菌国内外参考株核苷酸序列相似性为98.5%–100%,分离株在第609 bp处出现T突变为G、769 bp处C突变为A,对应的氨基酸第203位Ile突变为Met、第257位Leu突变为Ile,为Met-203、Ile-257型;分离株形成8个PFGE基因型,相似度达88.8%–100%,优势基因型为ER2(54.8%),弱毒疫苗G_4T_(10)和GC_(42)株独立为同一个基因型。【结论】江淮地区致病猪丹毒杆菌流行血清型为1a型,spa A基因相似性高,分离株变异小、源于同一克隆系,Met-203、Ile-257型菌株致病力强,是江淮地区猪丹毒发生与流行的主要致病菌型。
[Objective] To study the serotypes, phylogeny of surface protective antigen A gene（spa A）, and molecular subtyping of Erysipelothrix rhusiopathiae isolated between 2012 and 2015 in Jianghuai area. [Methods] We collected 42 clinically isolated Erysipelothrix rhusiopathiae strains. Agar gel precipitation tests, PCR and sequence analysis methods, and pulsed-field gel electrophoresis were used to investigate the serotypes, phylogeny of spa A gene, and PFGE typing, respectively. [Results] All 42 strains belonged to serotype 1a. spa A gene was 98.5% to 100% homologous to the reference strains. The sequences showed that the mutations occurred from T to G and C to A at base 609 th and 769 th respectively, and the corresponding amino acid showed mutations from Ile to Met as Met-203-type, and Leu to Ile as Ile-257-type strains; PFGE revealed 8 different genotypes, with 88.8% to 100% similarity. ER2（54.8%） was the dominant PFGE genotype, and erysipelas attenuated vaccine strains（G4T10 and GC42） were the same genotype strains. [Conclusion] Erysipelothrix rhusiopathiae serotype 1a was the prevalent serotypes in Jianghuai area. The homology of the spa A was high and had small variations. All the isolates came from one clone strain. Met-203-type and Ile-257-type strains had high pathogenicity and were the most prevalent pathogens type in Jianghuai area.
出处
《微生物学通报》
CAS
CSCD
北大核心
2017年第3期664-672,共9页
Microbiology China
基金
国家星火计划重点项目(No.2014GA710002)
安徽省质量工程项目(No.2013sxzx008)
安徽农业大学大学生创新基金项目(No.XJDC2014140)
安徽省生猪产业体系基金项目~~
关键词
猪丹毒杆菌
血清型
表面保护性抗原A基因
脉冲场凝胶电泳
Erysipelothrix rhusiopathiae
Serotypes
Surface protective antigen A gene
Pulsed-field gel electrophoresis
