摘要
目的研究紫外线(UVB)诱导人皮肤成纤维细胞中端粒长度及基质金属蛋白酶(MMPs)的变化,及其在UVB诱导光老化中的作用。方法原代培养人成纤维细胞,用第5代细胞进行UVB照射处理。观察细胞形态,实时定量PCR检测COL1a1和h TERT的mRNA表达细胞端粒长度,Western blot检测MMP-3和MMP-1蛋白。结果 30 m J/cm2UVB照射人成纤维细胞24 h后,细胞逐渐变圆、皱缩和排列紊乱;COL1a1和TERT的mRNA水平表达显著升高,基质金属蛋白酶MMP-3和MMP-1蛋白水平也显著升高,端粒长度显著缩短。结论 UVB可能诱导人皮肤成纤维细胞形态改变,及MMP-3和MMP-1的表达明显升高,启动了光老化的早期进程。
Objective To observe the changes of telomere length and MMPs level in human fibroblasts induced by UVB,and to explore their roles on skin photoaging. Methods Human skin fibroblasts were extracted and cultured. The 5th fibroblasts were irradiated by UVB. The morphology of fibroblasts were microscoped,and the length of telomere and the mRNA expression of COL1a1 and h TERT were detected by RT-q PCR. The expression of MMP-3 and MMP-1 were detected by Western blot. Results The fibroblasts gradually became round,wrinkled and disorderly arranged after 30 m J / cm^2 UVB irradiation for 24 h. The mRNA level of COL1a1 and h TERT and the expression of MMP-3 and MMP-1 were significantly increased after UVB irradiation compared with control,and the length of telomere was shortened. Conclusions UVB may frigger the early process of photoaging by the morphological changes of human skin fibroblasts and increasing the expression of MMP-3 and MMP-1.
出处
《基础医学与临床》
CSCD
2017年第3期360-363,共4页
Basic and Clinical Medicine
基金
黑龙江省卫生厅科研课题(2010-036)
