摘要
目的探讨枸杞多糖(LBP)对中波紫外线(UVB)辐射致体外培养人皮肤成纤维细胞损伤的保护作用。方法采用强度30mJ/cm2的中波紫外线照射体外培养的人皮肤成纤维细胞,实验分为对照组、紫外线照射模型组和四个不同浓度LBP保护组,用MTT法检测细胞增殖活性,酶生化法检测细胞内SOD活性、MDA含量,以及培养液中LDH漏出率。结果强度为30mJ/cm2的中波紫外线可以造成体外培养的人皮肤成纤维细胞增殖活性降低,细胞内SOD活性降低,MDA含量升高,LDH漏出增加,与对照组相比均P<0.05。给定浓度范围的LBP能剂量依赖性地增加细胞的增殖活性,增加细胞内SOD活性、MDA含量,减少LDH的漏出。结论紫外线可以造成体外培养的人皮肤成纤维细胞氧化损伤,LBP对UVB致人皮肤成纤维细胞的损伤具有一定保护作用,其机制可能与它的抗氧化作用和促进细胞增殖作用有关。
Objective To investigate the protective effects of Lycium Barbarum Polysaccharides（LBP） on fibroblast damaged by ultraviolet B（UVB）. Methods The fibroblast was irradiated by UVB （irradiation intensity：30mJ/cm^2） . The fibroblasts was randomly divided into six groups including 1 control group and 5 UVB radiated groups （radiated group, 0.1 mg/mL LBP, 0.2mg/mL LBP, 0.4 mg/mL LBP 0.8mg/mL LBP）. The enzymic activities were determined by MTT methods . The content of MDA 、 the activities of intracellular SOD, and the content of LDH in the supematants of cells were determined by biochemical methods. Results Compared to controls, UVB （irradiation intensity：30mJ/cm^2） can significantly decrease the proliferation activities in fibroblast and intracellular SOD and significantly increase MDA content and LDH. In the given concentration , LBP can dose-dependently significantly improve the activities of cellular proliferation and intracellular SOD and significantly decrease the contents of intracellular MDA and the content of LDH in the supematants of cells（ P 〈 0.01, respectively）. Conclusion The LBP has protective effect on fibroblast irradiated by UVB. The mechanism of the protective effect mav be associated with it＇s effect in antioxidative and enhancing celluar proliferation.
出处
《宁夏医学院学报》
2006年第6期501-503,共3页
Journal of Ningxia Medical College
基金
教育部科学技术研究重点项目(项目编号:02159)
