摘要
目的观察益母草总生物碱提取物对大、小鼠急性毒性反应。方法采用经典急性毒性实验法,进行益母草总生物碱提取物对大、小鼠的急性毒性研究。结果按含总生物碱含量计算,益母草总生物碱提取物对小鼠的LD50为5.707 2 g·kg^(-1)·d^(-1),95%的可信限为5.548 3~5.871 1 g·kg^(-1)·d^(-1)。益母草总生物碱提取物对大鼠的LD50为4.510 2 g·kg^(-1)·d^(-1),95%的可信限为4.159 5~5.010 2 g·kg^(-1)·d^(-1),死亡动物出现俯卧昏睡、怠动、呼吸急促、抽搐等症状。结论益母草总生物碱提取物对大、小鼠可造成急性毒性损伤,其产生毒性损伤的部位和毒性机制有待于深入研究。
Objective To observe the acute toxicity of total alkaloids extracts from Herba leonuri in mice and rats separately. Methods The classical method of acute toxicity was used to research the acute toxicity in mice and rats. Results Calculated by total alkaloids of Herba leonuri, the LD50 of the extracts in mice and rats were respectively 5.707 2 and 4.510 2 g·kg^（-1）·d^（-1）;95% confidence limits were respectively 5.548 3~5.871 1 and 4.159 5~5.010 2 g·kg^（-1）·d^（-1）. The toxic symptoms of the mice and rats after given extracts by ig were abdominal lying sleeping, hypokinesia, shortness of breath, convulsions in the experiment. Conclusion Ig the total alkaloids extracts from Herba leonuri to mice and rats can cause toxical injury to them, and its toxical location and mechanism remain to be further studied.
出处
《中国药物警戒》
2017年第1期1-3,9,共4页
Chinese Journal of Pharmacovigilance
基金
国家自然科学基金项目(81450021)
山东省自主创新和成果转化项目(2014ZZCX02104)
山东省泰山学者工程专项经费资助(ts 201511107)
关键词
益母草
总生物碱
大鼠
小鼠
急性毒性
Herba leonuri
total alkaloids
rat
mice
acute toxicity
