摘要
目的:探究白藜芦醇(RES)对脂多糖(LPS)诱导的人牙周膜细胞(hPDLCs)损伤的保护作用。方法:体外培养并鉴定hPDLCs,将培养的hPDLCs随机分为5组:对照组、LPS(10μg/ml)+RES(0、30、60、90μmol/L)组,MTT法检测各组细胞增殖能力,ELISA检测各组细胞分泌TNF-α/IL-6水平,PCR和Western blot检测各组细胞TLR4/NF-κB mRNA和蛋白表达。结果:分离培养的hPDLCs抗波丝蛋白表达阳性,抗角蛋白表达阴性。与对照组比,LPS处理后细胞增殖能力明显降低,细胞分泌TNF-α/IL-6水平和TLR4/NF-κB mRNA和蛋白表达明显增加;30~90μmol/L白藜芦醇预处理后,细胞增殖能力增加(P<0.05),细胞分泌TNF-α/IL-6水平、TLR4/NF-κB mRNA以及蛋白表达则下调(P<0.05),均呈现一定的浓度依赖性。结论:白藜芦醇可抑制TLR4/NF-κB活化并减轻LPS诱导的牙周膜细胞损伤。
Objective： To investigate the protective effect of resveratrol on lipopolysaccharide （LPS）- induced cell injury in human per- iodontal ligament cells （hPDLCs）. Methods： hPDLCs were cultured and identified. The cultured hPDLCs were divided into 5 groups： control group and LPS（ 10 μg/ml） ＋ RES（0/30, 60 and 90 μmol/L respectively） groups. The cell proliferation was detected by MTr assay. The secretion of TNF-α and IL-6 of hPDLCs was detected by ELISA kit. The expression of TLR4/NF-κB mRNA and protein was determined by PCR and Western blot analyses, respectively. Results： The cultured cells were negative for cytokeratin and positive for vimentin staining. Compared with the control group, cell proliferation was decreased, the secretion of TNF-α/IL-6 levels and the ex- pression of TLR4/NF- κB mRNA and protein were increased after treatment with LPS. Whereas, with 30 - 90 μmol/L resveratrol pre- treatment, the proliferation ability of hPDLCs was enhanced（ P 〈 0.05）, the secretion levels of TNF-α and IL-6 and the expression of TLR4/ NF-κB mRNA and protein were reduced（P 〈 0.01 ） in a dose-dependent manner. Conclusion： Resveratrol may attenuate LPS- induced cell injury by inhibiting TLR4/NF-κB pathway in hPDLCs.
作者
呼海燕
刘彩宏
HU Haiyan LIU Caihong.(716000, Repair Department of Stomatology, Yanan University Affiliated Hosptial, China)
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2017年第1期88-91,共4页
Journal of Practical Stomatology
