摘要
目的:研究不同环境因素对粪肠球菌侵入人工微管系统能力的影响和微流芯片的适用性。方法:采用软光刻技术制作微流芯片,观察细菌在牛脑心浸出液(BHI)、PBS和pH 10的情况下在微流芯片中的生长情况,测定和比较细菌在相应微管内的最大侵入深度。结果:粪肠球菌在营养缺乏和碱性环境下生长速度明显下降,侵入小管径微管的深度显著下降(P<0.01)。结论:营养缺乏和碱性环境可显著降低粪肠球菌侵入微管系统的能力。微流芯片具有高通量、平行对比和可观察性好等特点,有望为今后相关研究提供标准化的实验平台。
Objective： To evaluate the effects of different growth conditions on E. faecalis growth in the microfluid chip and the pene- tration of E. faecalis into microtubes. Methods： Four units on the chip were randomly selected as control, BHI, nutrient-Tdeprived （PBS） and pH 10 groups. The growth of E. faecali~ was monitored by microscope for a period of 72 h after the suspension of E. faecalis had been added into the chip. Results： The microscopic analysis showed a distinct variation in the growth rate and morphological feature under different experimental conditions, he depth of bacterial penetration was significantly greater in BHI group. Conclusion： This study demonstrated that environmental changes can significantly influence the growth and penetration of E. faecalis into the micro tubes.
作者
孙晓强
王淑静
罗春雄
侯本祥
SUN Xiaoqtang WANG Shujing LUO Chunxiong HOU Benxiang(100050, Department of Endodontics, School of Stomatology Capital Medical University Beijing, China State Key Laboratory for Artificial Microstruc- tures and Mesoscopic Physics, School of Physics Peking University Beifing)
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2017年第1期49-53,共5页
Journal of Practical Stomatology
基金
国家自然科学基金(编号:81170952,11174012,11434001)
北京市医院管理局临床医学发展专项经费资助(编号:XM-LX201301)
关键词
牙本质小管
粪肠球菌
微流芯片
持续性根尖周炎
Dentinal tubules
Enterococcus faecalis
Microfluidics
Persistent apical periodontitis
