摘要
目的探讨饥饿环境对粪肠球菌生物膜胞外多糖合成能力的影响。方法体外培养饥饿状态粪肠球菌形成48h生物膜,凝集素标记结合倒置荧光显微镜观察胞外多糖分布情况,提取饥饿状态浮游粪肠球菌和对数期、稳定期、饥饿状态粪肠球菌48h生物膜胞外多糖,蒽酮法定量分析比较胞外多糖合成能力。结果饥饿状态粪肠球菌形成的48h生物膜内胞外多糖呈云絮状,与细菌分布区域不完全一致;生物膜细菌合成水溶性和水不溶性胞外多糖的能力均高于浮游细菌(P<0.05);其合成水溶性胞外多糖的能力与对数期和稳定期细菌无显著差别(P>0.05),合成水不溶性胞外多糖的能力高于后两者(P<0.05)。结论饥饿状态粪肠球菌合成生物膜胞外多糖的能力增强。
Objective To investigate the influence of starvation on the ability of extracellular polysaceharide synthesis by Enterococcus faecalis （E.faecalis） biofilm-grown cells. Methods E.faecalis in starvation phase was developed to form biofilm. Fluorescent stains and inverted fluorescence microscope were used to monitor the distribution of extracellular polysaceharide in biofilm. Anthrone method was used to quantify the amount of extracellular polysaccharide in planktonic E.faecalis and the biofilms developed by E.faeca!is cells in exponential, stationary and starvation phases. Results Extracellular polysaccharide was observed in 48 h biofilm formed by E.faecalis cells in starvation phase. The water soluble and water insoluble polysaccharide in the biofilm were significantly higher than those in planktonic cells （P〈 0.05）. The differences of the amount of water soluble polysaccharide among the biofilms developed by cells in different physiologic states were not significant （P〉 0.05）. The water insoluble polysaceharide in the biofilm formed by starved cells was higher than those formed by cells in exponential and stationary phases （P 〈 0.05 ）. Conclusion The ability of extraeellular polysaccharide synthesis by E.faecalis biofilm-grown cells increased significantly in nutrient-poor environment.
出处
《中华口腔医学研究杂志（电子版）》
CAS
2012年第5期16-20,共5页
Chinese Journal of Stomatological Research(Electronic Edition)
基金
卫生部部属(管)医院2010-2012年度临床学科重点项目(显微牙髓治疗导航技术提高再处理患牙疗效的研究)
广东省自然科学基金博士启动项目(S2011040002747)
关键词
粪肠球菌
生物膜
胞外多糖
饥饿
Enterococcus faecalis
Biofilm
Extracellular polysaccharide
Starvation
