摘要
目的比较快速生物指示剂与常规生物指示剂对环氧乙烷灭菌监测效果,寻找环氧乙烷灭菌效果快速检测方法。方法通过环氧乙烷生物指示剂抗力评估系统,对两种培养法生物指示剂监测环氧乙烷灭菌效果比较。结果在相同环氧乙烷灭菌设定条件下,灭菌20 min和120 min,两种方法培养生物指示剂的结果一致;70 min灭菌时段,两种培养法持续培养48 h结果一致,但快速培养法在培养4 h的结果与培养48 h的结果不一致。结论环氧乙烷生物监测快速培养法相比常规培养法,拥有更快速评判灭菌效果的能力,但可能存在假阳性的结果。
Objective To compare the efficacy of estimating ethylene oxide（ EO） sterilization performance with rapid readout biological indicator for EO test pack and normal EO test pack in order to finding substitute for shorten the estimating time. Methods The two packs were evaluated by using methods of EO biological indicators for sterilization through biological indicator evaluator resistometer（ BIER）. Results In 20 min and 120 min intervals the results of two methods were the same. In 70 min interval the results of two methods culturing for 48 h were the same,but the result of rapid readout method for EO test pack culturing for 4 h was different with the result of itself culturing for 48 h. Conclusion It is possible that false positive results exist in rapid readout methods for EO test pack,which is faster than normal method to judge the sterilization effect.
作者
吴晓松
王嵬
陈越英
张伟
谈智
王晓蕾
王玲
褚宏亮
周明浩
徐燕
WU Xiao - song WANG Wei CHEN Yue - ying ZHANG Wei TAN Zhi WANG Xiao - lei WANG Ling CHU Hong -liang ZHOU Ming -hao XU Yan(Jiangsu Provincial Center for Disease Prevention and Control, Nanjing Jiangsu 210009, China)
出处
《中国消毒学杂志》
CAS
北大核心
2016年第12期1149-1151,共3页
Chinese Journal of Disinfection
基金
江苏省社会发展科技示范工程(BE2015714)
江苏省"十二五"科教兴卫工程(ZX201109)
关键词
环氧乙烷
灭菌效果监测
生物指示剂
快速培养法
常规培养法
ethylene oxide
monitoring sterilization effect
biological indicator
rapid readout method
normal method
