摘要
目的探讨micro RNA-22在冠状动脉粥样硬化性心脏病(冠心病)患者外周血中的变化水平及其临床价值,同时分析其与氧化应激的关系。方法纳入于本院就诊的具有心血管疾病风险并考虑为冠心病的患者96例作为研究对象,其中男59例,女37例,年龄(62±8)岁。根据影像学检查结果,将研究对象分为冠心病组及非冠心病组。采集研究对象血浆标本,实时荧光定量PCR(quantitative real-time,PCR)检测micro RNA-22水平。Diacron-活性氧代谢产物检测试剂盒检测血浆活性氧代谢产物(reactive oxygen metabolites,ROM)水平。分析micro RNA-22与冠心病发病、临床表现以及病情复杂程度(SYNATAX评分)的关系。结果冠心病组患者血浆中的micro RNA-22高于非冠心病组,且差异具有显著性(P<0.05)。临床表现为ST段抬高型心肌梗死的患者血浆micro RNA-22水平高于其他表现的患者(P<0.05)。冠心病病变较复杂(高SYNATAX评分)的患者micro RNA-22水平较高(P<0.05)。冠心病组患者的血浆ROM高于非冠心病组,差异具有显著性(P<0.05)。冠心病患者血浆micro RNA-22与ROM水平成正比(r=0.641,P<0.05)。高血浆micro RNA-22水平是冠心病发病的危险因素(OR=2.98,95%CI:1.82~4.87,P<0.05),受试工作者曲线提示血浆micro RNA-22诊断冠心病的曲线下面积为0.714。结论外周血micro RNA-22在冠心病中存在高表达,可用于评估冠心病的病情及发病风险,具有临床诊断价值,同时冠心病患者的外周血micro RNA-22与氧化应激水平有关。
Objective To investigate the level change and clinical significance of micro RNA-22 in the diagnose of coronary atherosclerotic heart disease（CHD） and its relationship with oxidative stress. Method 96 subjects with cardiovascular risk and suspected CHD were enrolled in the present study including 59 males and 37 females with the average age of（62±8） years. According to the CT and angiography results, all subjects were divided into two group including CHD group and non-CHD group. The venous blood samples were collected and plasma samples were prepared. Using q RT-PCR detected the level of micro RNA-22 in all subjects. To detect the reactive oxygen metabolites（ROM）, a Diacron- reactive oxygen metabolites kit was used. Result Compared with the patients without CHD, the micro RNA-22 level in patients with CHD was higher（P〈0.05）. The micro RNA-22 level was different in patients with different clinical type o CHD or different SYNTAX score. Compared with non-CHD group, the ROM level was higher in CHD group（P〈0.05）, moreover, the micro RNA-22 level was proportional to ROM level in CHD patients（r=0.641, P〈0.05）. Higher micro RNA-22 level was a risk factor for CHD（OR=2.98,95%CI： 1.82~4.87, P〈0.05）. Receiver operating curves showed that the area under the curve of micro RNA-22 was 0.714 in diagnose of CHD. Conclusion Plasma micro RNA-22 could be used as a biomarker in risk estimation in CHD and also has clinical diagnose value. There is an association between peripheral serotonin and oxidative stress.
作者
李陶
庞琪
刘永斌
LI Tao PANG Qi LILT Yong-bin(Department of cardiovascular, Lanzhou Petrochemical General Hospital in Gansu Province, Gansu Lanzhou 730060, China Respiratory Medicine, Lanzhou Petrochemical General Hospital in Gansu Province, Gansu Lanzhou 730060, China)
出处
《中国医刊》
CAS
2017年第1期31-35,共5页
Chinese Journal of Medicine
