摘要
为了获得广西陆川猪激素敏感性脂肪酶(HSL)基因编码区序列(CDS),试验从11月龄陆川猪背最长肌组织中提取总RNA,利用反转录-聚合酶链式反应(RT-PCR)方法分段扩增出HSL基因CDS。结果表明:成功分段克隆出陆川猪HSL基因CDS,拼接后长度为2 295 bp,与Gen Bank数据库中公布的国内地方猪种梅山猪的同源性为99.9%,与外来猪种长白猪的同源性为99.7%,并发现其1 044位点存在C→T,其碱基T是陆川猪所特有,尚未导致氨基酸突变。利用生物软件构建系统进化树显示,与广西陆川猪遗传距离最近的是梅山猪,它们聚为同一支,最远的是人。陆川猪HSL基因的二级结构包含α-螺旋、β-折叠。
To obtain the coding sequence （CDS） of hormone- sensitive lipase （HSL） gene from Luehuan pig in Guangxi, total RNA was ex- tracted from the Longissimus dorsi tissue of 11 - month - old Luchuan pig, and the CDS fragments of HSL gene were amplified by reverse tran- scription -polymerase chain reaction （RT -PCR）. The results showed that the CDS fragments of HSL gene were successfully cloned, and the length of the CDS was 2 295 bp after splicing. The CDS of HSL gene from Luchuan pig shared 99.9% and 99.7% homology with that from the local breed of domestic Meishan pig and the foreign breed Landrace published in GenBank, respectively. It was found that there was a mutation from C to T at 1 044 locus, and its base T was unique to Luchuan pig and didnt cause amino acid mutation. A phylogenetic tree constructed by biological software showed that Luchuan pig in Guangxi was genetically closest to Meishan pig, both were clustered into the same branch, while Luchuan pig in Guangxi was genetically farthest to human. The secondary structure of HSL gene from Luchuan pig containedα - helix and β - fold.
出处
《黑龙江畜牧兽医》
CAS
北大核心
2017年第1期98-101,294,共5页
Heilongjiang Animal Science And veterinary Medicine
基金
国家自然科学基金项目(31460606)
广西自然科学基金项目(2013GXNSFBA019077
2014GXNSFAA118131)
广西高校科研一般项目(2013YB004)
