摘要
目的:探讨丹皮酚、三七总皂苷联合应用对心肌梗死后心室重构大鼠心肌组织中肿瘤坏死因子(TNF-α)表达的影响。方法:结扎左冠状动脉前降支制备急性心肌梗死大鼠动物模型;将存活且造模成功的大鼠随机分成模型组、丹皮酚组、三七总皂苷组、丹皮酚与三七总皂苷联合用药低剂量组、高剂量组和卡托普利组,另立假手术组。连续给药4周,Masson染色检测各心肌组织胶原容积分数(CVF),Western-blot检测心肌组织中TNF-α蛋白的表达水平。结果:与模型组比较,各用药组CVF和TNF-α蛋白表达不同程度降低;与单味药丹皮酚组和三七总皂苷组比较,联合用药组CVF和TNF-α蛋白表达下调显著,联合用药高剂量组较联合用药低剂量组作用更显著。结论:丹皮酚与三七总皂苷联合应用可协同下调TNF-α蛋白表达,改善心室重构。
Objective：To investigate the effects of paeonol（Pae） combined with PNS on TNF-α expression in rats with ventricular remodeling after acute myocardial infarction（AMI）. Methods：AMI model was made by ligaturing left descending coronary artery. Alive rats with AMI were randomly divided into model group,Pae treated group,PNS treated group,Pae plus PNS treated groups and the positive control group with captopril and the sham operation group. Collagen volume fraction（CVF） by Masson staining and expression of TNF-αwas detected by Western blot assay after four-week,treatments in all groups. Results：Compared with the model group,CVF and the levels of TNF-αreduced to various extents. In Pae plus PNS groups,CVF was lower and the expression of TNF-αwas down-regulated more than in Pae or in PNS group. the effects were more significant in combination of high-dose group than low-dose group. The inhibiting effects were more significant when Pae and PNS were given at higher doses. Conclusions：The combination of Pae and PNS can play an obvious synergistic action in inhibiting ventricular remodeling in AMI rats by down-regulation of TNF-αexpression.
出处
《天津药学》
2016年第6期9-11,共3页
Tianjin Pharmacy
基金
河北省自然科学基金项目(No.C2011406009)
河北省教育厅重点项目(No.ZD20131068)
