摘要
目的探讨交通相关细颗粒物对CEM-6T细胞凋亡的影响,为研究交通相关细颗粒物的免疫毒性机制提供实验依据。方法使用不同剂量(0、20、80、320μg/ml)交通相关细颗粒物染毒CEM-6T细胞24和48 h,用RT-PCR测定CEM-6T细胞中Caspase-3的mRNA表达,以Western Blot测定Caspase-9、Cyt-c、Fas-L、Caspase-8和TNF-α蛋白表达。结果 320μg/ml交通相关细颗粒物染毒细胞24 h和48 h后,Caspase-3基因表达高于对照组,差异有统计学意义(P<0.05)。80、320μg/ml细颗粒物染毒细胞24和48 h后,细胞内Cyt-C、Caspase-9、Fas-L、Caspase-8、TNF-α蛋白表达升高,与对照组比较,差异有统计学意义(P<0.05)。结论交通相关细颗粒物可通过线粒体通道和死亡受体通道诱导CEM-6T细胞凋亡。
Objective To investigate the effect of traffic-related fine particulate matter on CEM-6T cell apoptosis and provide experimental basis for the immunotoxieity mechanism. Methods CEM-6T cells were exposed to different doses of traffic-related fine particulate matter （0, 20, 80 and 320 μg/ml） for 24 h and 48 h respectively. The mRNA expression of Caspase-3 was determined by RT-PCR. The protein expression of Caspase-9, Cyt-c, Fas-L, Caspase-8 and TNF-α were detected by Western Blot. Results CEM-6T cells were exposed to 320 μg/ml of traffic-related fine particulate matter for 24 h and 48 h, the Caspase-3 mRNA expression increased significantly compared with saline control group （P〈0.05）. When the cells were exposed to 80 and 320 μg/ml of fine particulate for 24 h and 48 h, the protein expression of Cyt-c, Caspase-9, Fas-L, Caspase-8 and TNF-α enhanced, the differences with the saline control group were statistically significant（P〈0.05）. Conclusion Traffic-related fine particulate matter may induce CEM-6T apoptosis through the mitochondrial and death receptor pathway.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2011年第12期1062-1064,共3页
Journal of Environment and Health
基金
国家自然科学基金(30700655)
2009年度山西省高等学校优秀青年学术带头人支持项目
