摘要
目的建立大鼠急性低压缺氧模型,探讨急进高原大鼠视网膜形态学变化及白藜芦醇对大鼠视网膜低氧损伤的保护作用及机制。方法将54只健康SD大鼠随机分成3组:常氧对照组、低氧模型组、低氧+白藜芦醇干预组。常氧对照组大鼠在常氧环境下正常喂养(西宁,海拔2260 m),低氧模型组及低氧+白藜芦醇干预组大鼠置于低压氧舱内(模拟5000 m的海拔高度),每日持续24 h。白藜芦醇干预组每日腹腔注射白藜芦醇(30 mg/kg,每日1次)。7 d后剥离视网膜。HE染色,光镜下观察大鼠视网膜组织形态;酶联免疫吸附测定法(ELISA)测定大鼠视网膜组织中过氧化物歧化酶(Superoxide Dismutase,SOD)、谷胱甘肽过氧化物酶(Glutathione peroxidase,GSH-PX)的活性。RT-PCR方法检测低氧诱导因子(hypoxia inducible factor,HIF)mRNA表达。结果与常氧对照组相比,光镜下低氧模型组大鼠视网膜形态发生明显变化;低氧模型组大鼠视网膜组织中GSH-PX活性显著下降(P<0.05),SOD活性有所下降,但差异无统计学意义(P>0.05),HIF mRNA表达量显著增加(P<0.01);低氧+白藜芦醇干预组GSH-PX、SOD活性有所下降,HIF mRNA表达量轻度减少,差异无统计学意义(P>0.05)。与低氧模型组相比,低氧+白藜芦醇干预组GSH-PX活性增强(P<0.05),SOD活性升高,但差异无统计学意义(P>0.05);HIF mRNA表达量明显减少(P<0.01)。结论急性低压缺氧环境影响大鼠的视网膜功能,而白藜芦醇对此损伤有干预作用,可能与其增强SOD与GSH-PX的酶活性,增强对自由基的清除以及调节HIF的表达有关。
Objective To investigate the influence of hypobaric hypoxia on the retina by establishing the model of hypobaric hypoxia, and study the protective effects of resveratrol on rat retina hypoxic damage and explore the mechanism involving the pathlogical process.Methods 54 SD rats were randomly divided into the control group ,hypoxic group and resveratrol intervention group.The control croup was feeding at 2260 m above sea level,the hypoxia group and the resveratrol intervention group were maintained at low pressure oxygen cabin（ simulation for 5000 m a- bove sea level）for a full 24 hours a day. Resveratrol intervention group was given daily intraperitoneal injection （30 mg/kg,once a day）.Retinal tissue in each group was gentlely removed after rats were treated for 7 days and tested by HE staining.The levels of superoxide dismutase （SOD）and Glutathione peroxidase （GSH-PX）in retina were detected by enzyme-linked immunosorbent assay （ ELISA）.The expression of hypoxia inducible factor （HIF） was evaluated by RT-PCR. Result Compared with control group, the obvious pathological changes were shown in retina of hypobaric hypoxia model group ; the level of GSH-PX（P〈0.05） and SOD（P〉O.05） decreased, whereas, the HIF mRNA expression was upregulated （ P 〈 0.01 ） in this group. In Resveratrol intervention group （ P 〉 0.05 ）, the GSH-PX level, SOD activity and HIF mRNA expression in Resveratrol intervention group were decreased, but there were no statistical difference（P〉0.05）in comparison with normal control group.Compared with hypobaric hypoxia model group, the activity of GSH-PX was increased （ P〈0.05 ） in Resveratrol intervention, and the increased SOD level also presented in this group ,but there was no significant difference（P〉0.05） ;the HIF mRNA expression was decreased in Resveratrol intervention group compared with hypobaric hypoxia group, significant differences was observed between the two groups（P〈0.01 ）.Conclusion Acute hypobaric hypoxia may cause the pathological changes of rat retina.The intervention of resveratrol shows a protective role on acute hypobaric hypoxia induced retina impairment by increasing antioxidant production including SOD and GSH-PX.Meanwhile it can also enhance the ability to remove the free radicals and regulating the expression of HIF.
作者
党鸿
辛晓蓉
DANG Hong XIN Xiao-rong(Qinghai University Medical College Qinghai Red Cross Hospital)
出处
《青海医学院学报》
CAS
2016年第4期217-221,共5页
Journal of Qinghai Medical College
基金
青海省自然科学基金项目(编号:2014-Z-911)
