摘要
红麸杨果实经纤维素酶处理后,采用体积分数70%乙醇-大孔吸附树脂联用技术对黄颜木素进行提取、纯化,以黄颜木素得率为考察指标,采用正交试验优化了酶辅助提取工艺,并从8种大孔吸附树脂中筛选出对黄颜木素分离纯化最佳的树脂,研究了该树脂对黄颜木素静态、动态吸附与解吸效果。结果表明:酶处理的最佳条件为酶用量120 U/mL,酶解温度55℃,酶解时间60 min,酶解pH值4.5,黄颜木素得率可达1.35%,纯度为14.59%。通过静态吸附试验筛选出最有效的XDA-8树脂用于黄颜木素的纯化,XDA-8分离纯化黄颜木素动态吸附试验的最佳条件为上样质量浓度14.4 g/L,流速80.384 mL/h,洗脱液乙醇体积分数为60%,洗脱液流速80.384 mL/h,解吸率为91.86%,纯化后黄颜木素纯度达到了68.15%。
After the Rhus punjabensis var. sinica fruits were disposed by cellulase, the volume fraction of 70% ethanolmacroporous resin combined technique was used to extract and purify fustin. With the fustin yield as study index, the orthogonal experiment was employed to optimize the extraction process. The best resin for fustin separation and purification was screened from eight kinds of macroporous resin to study the effect on the static and dynamic adsorption and desorption of fustin. The results showed that the optimum conditions for the enzyme treatment were enzyme dosage 120 U/mL, enzymolysis temperature 55 ℃, enzymolysis time 60 min and enzymolysis pH value 4.5, and the fustin yield was up to 1.35% with the purity of 14.59%. Through Static adsorption test, the most effective XDA-8 resin was selected. The dynamic adsorption test showed that the best conditions for XDA-8 separation and purification were sample mass concentration of 14. g g/L, flow rate of 80. 384 mL/h, ethanol volume fraction of 60% and eluent flow rate of 80.384 mL/h. Under these conditions, the desorption rate was 91.86% and after purification the fustin purity could reach 68.15%.
出处
《林产化学与工业》
EI
CAS
CSCD
北大核心
2016年第6期100-106,共7页
Chemistry and Industry of Forest Products
基金
黔教合重大专项(字[2012]018号子项301号)
张家界市科技局项目(2015FJ1149)
吉首大学校级科研项目(15JDY018)
关键词
红麸杨果实
纤维素酶
黄颜木素
大孔吸附树脂
HPLC
Rhus punjabensis var. sinica Rehd. fruit
cellulase
dihydrofisetin
macroporous adsorption resin
HPLC
