摘要
应用本实验室制备的对沙丁胺醇（SAL）具有高亲和力的多克隆抗体和酶标半抗原,采用包被抗体-酶标半抗原直接竞争模式建立沙丁胺醇的酶联免疫吸附分析（ELISA）法。在优化实验条件下,ELISA法检测沙丁胺醇的线性浓度范围为0.11.0×103μg/L,沙丁胺醇对抗体-酶标半抗原结合反应的抑制中浓度（Ic50）为14μg/L,相对标准偏差（RSD）为8.6%（n=5）,定量检测下限（Ic10）为0.29μg/L。在猪饲料中分别添加沙丁胺醇标样10、50、250μg/kg,ELISA法检测的回收率分别为85%108%、81%92%和81%102%,RSD（n=5）分别为9.1%、5.6%和8.9%,对饲料中沙丁胺醇的最低定量检测浓度为4.23μg/kg。利用高效液相色谱-紫外检测（HPLC-UV）法同步测定饲料中添加250μg/kg沙丁胺醇的平均回收率为89%（n=3）,相对标准偏差为3.9%。
The polyclonal antibody with high affinity to salbutamol（SAL）and enzyme labelled hapten prepared in our lab were employed to develop the antibody immobilized direct competitive enzyme-linked immunosorbent assay（ELISA） for monitoring SAL in pig feed. The result showed that the linear concentration for SAL analysis ranged from 0.1 μg/L to 103 μg/L, the half-maximal inhibitory concentration（Ic50 ）of SAL to the combination of antibody and enzyme labelled hapten was 14μg/L with the relative standard deviation（ RSD） of 8.6% （n= 5）, the quantitative detection limit（Icl0 ） was 0. 29 μg/L. The SAL recoveries in pig feed determined by ELISA were 85%-108%,81%-92% and 81%-102% with the RSD （n=5） of 9.1%,5.6% and 8. 9% at the spiked levels of 10,50 and 250μg/kg,respectively. At the mean time,SAL in pig feed was also determined by HPLC-UVD, and the recovery were 89% at the spiked level of 250μg/kg,RSD=3.9%.
出处
《分析科学学报》
CAS
CSCD
北大核心
2016年第6期774-778,共5页
Journal of Analytical Science
基金
国家自然科学基金(No.21247002)
国家863专题(No.2009AA03Z331)
关键词
沙丁胺醇
饲料
直接竞争ELISA
高效液相色谱
Salbutamol
Feed
Direct competitive ELISA
High performance liquid chromatography
