摘要
目的:建立不同等级延胡索药材HPLC指纹图谱,并同时测定原阿片碱、盐酸巴马汀、盐酸小檗碱、去氢紫堇碱、延胡索乙素和紫堇碱6种主要成分的含量,对不同等级延胡索药材质量进行研究。方法:对延胡索药材等级分类,采用Agilent Eclipse XDB-C18(4.6μm×250 mm,5μm),乙腈-0.6%醋酸溶液(p H5.0)为流动相进行梯度洗脱,检测波长280 nm。对10批3个等级延胡索药材进行测定,运用国家药典委员会"中药色谱指纹图谱相似度评价软件"2004A版进行评价,并对6种成分进行含量测定。结果:含量测定条件通过方法学验证,精密度、线性关系、准确度、稳定性、重现性良好;建立了延胡索生物碱类成分HPLC指纹图谱。结论:该法所建立的延胡索生物碱类成分指纹图谱特征性强、方法简便,结合6个生物碱含量测定可更好地控制其质量,对延胡索等级分类具有指导意义和参考价值。
Objective： To establish a method for HPLC fingerprint determination of different grade of Rhizoma corydalis and simultaneous determine the contents of Protopine,Palmatine hydrochloride,Berberine hydrochloride,Dehydrocorydaline,Tetrahydropalmatine and Corydaline in Rhizoma corydalis and further study the quality standard of different grades of Rhizoma corydalis.Methods： With the different grades of Rhizoma corydalis,the optimal conditions of separation and detection were achieved on a Agilent Eclipse XDB-C18（4.6 μm × 250 mm,5 μm） with linear gradient elution of acetonitrile-0.6% acetate solution（p H = 5.0）.The detection wavelength was 280 nm.Collect 10 batches 3 grades of Rhizoma corydalis to assess the similarities according to similarity for Chromatographic Fingerprint of Traditional Chinese Medicine（2004A）.Results： Chromatography condition on determining the alkaloids contents in Rhizoma corydalis by HPLC was advantageous.The HPLC fingerprint of different grades of Rhizoma corydalis was established.Conclusion： This study indicated that the combination of quantitative and chromatographic fingerprint analysis can be readily utilized as the quality control method for different graded of Rhizoma corydalis.
出处
《中华中医药学刊》
CAS
北大核心
2016年第12期2868-2874,共7页
Chinese Archives of Traditional Chinese Medicine
基金
国家中医药行业科研专项项目(201507002)
关键词
延胡索
等级
生物碱
相似度
指纹图谱
Rhizoma corydalis
grade
alkaloids
similarity
fingerprint
