摘要
目的建立直接进样-超高效液相色谱-串联质谱法测定生活饮用水中莠去津及其代谢产物的残留。方法水样经0.22μm滤膜过滤后直接进样,超高效液相色谱串联三重四级杆质谱分析;以ACQUITY UPLC BEH C18(2.1 mm×50 mm,1.7μm)色谱柱分离,乙腈-水溶液为流动相进行梯度洗脱,采用正离子扫描多反应监测(MRM)模式检测。结果莠去津及其代谢产物在各自的考察浓度范围内线性关系良好(r≥0.999 7)。方法的检出限为0.000 5μg/L^0.04μg/L,莠去津及其代谢产物在出厂水和水源水中的加标回收率分别为81.4%~104.3%、74.7%~103.7%,相对标准偏差(n=7)分别为2.3%~6.6%、3.0%~8.0%。结论该方法操作简单、快捷,灵敏度和准确度均较高,精密度较好,适用于生活饮用水中莠去津及其代谢产物残留的测定。
Objective To establish a method for the determination of atrazine and its four metabolites in drinking water with direct injection- ultra high performance liquid chromatography- tandem mass spectrometry（ UPLC- MS / MS）. Methods The water samples were filtered by 0. 22 μm filter membrane and directly analyzed by UPLC- MS / MS using ACQUITY UPLC BEH C18（ 2. 1 mm × 50 mm,1. 7 μm）. Acetonitrile water solution was used as mobile phase for gradient elution. Multiple reaction monitoring（ MRM） mode is used in mass spectrometry acquisition. Results Good linearity was obtained when the concentrations of atrazine and its 4 metabolites were within the standard range（ r≥0. 999 7）. Method detection limit was within 0. 000 5 μg / L-0. 04 μg / L. The spiked recoveries of the analytes in tap water and source water were within 81. 4%- 104. 3% and 74. 7%-103. 7%,respectively,and the corresponding relative standard deviations（ RSD,n = 7） were within 2. 3%- 6. 6% and3. 0%- 8. 0%,respectively. Conclusion The method is simple,rapid,sensitive and accurate with higher precision. It is applicable to determinate atrazine and its metabolites in drinking water simultaneously.
出处
《中国卫生检验杂志》
CAS
2016年第21期3069-3071,共3页
Chinese Journal of Health Laboratory Technology
关键词
莠去津
代谢产物
饮用水
超高效液相色谱-串联质谱法
Atrazine
Metabolites
Drinking water
Ultra performance liquid chromatography-tandem mass spectrometry
