摘要
目的观察电针内关穴对心肌肥厚大鼠缺血心肌原癌基因丝氨酸/苏氨酸蛋白激酶-1(protooncogene serine/threonine-protein kinase-1,Raf-1)、细胞外信号调节激酶1/2(extracellular signal regulated kinase 1/2,ERK 1/2)和磷酸化细胞外信号调节激酶1/2(phospho-extracellular signal-regulated kinase 1/2,p-ERK 1/2)蛋白表达的影响。方法清洁级健康SD大鼠40只按随机数字表法分为正常组、模型组、对照组和电针组,每组10只,除正常组正常饲养外,其余三组采用颈背部皮下注射盐酸异丙肾上腺素[isoprinosine hydrochloride,ISO,3 mg/(kg·d)]制备大鼠左心室心肌肥厚模型,共14天。电针组每天注射ISO后取"内关"穴治疗,采用连续波,频率2 Hz,强度1 m A,通电20 min,1次/天,共14天。对照组选取非穴位,内关穴外侧旁开5 mm,干预方法与电针组相同。干预结束后观察大鼠心电图并称重,麻醉后开胸摘取心脏并称重,然后分离左心室称重,并计算左心室质量指数(left ventricular weight index,LVWI)和全心质量指数(heart weight index,HWI);免疫印迹法检测左心室心肌组织Raf-1、p-ERK 1/2及ERK 1/2蛋白含量。结果与正常组比较,模型组ST段抬高幅度、HWI、LVWI、心肌组织Raf-1及p-ERK 1/2蛋白表达升高(P<0.01)。与模型组及对照组比较,电针组ST段抬高幅度、HWI、LVWI、Raf-1及p-ERK 1/2蛋白表达降低(P<0.05)。结论电针能有效调节心肌肥厚大鼠心肌缺血状况,降低心肌肥厚大鼠心脏指数、Raf-1及p-ERK 1/2蛋白含量,可能是电针通过调节Raf/MEK/ERK改善心肌肥厚的信号调节机制之一。
Objective To observe the effects of electroacupuncture （EA） at Neiguan （PC6） on protein expressions of proto-oncogene serine/threonine-protein kinase-1 （Raf-1）, phospho-extracellular signal-regulated kinase 1/2 （p-ERK 1/2）, and extracellular signal-regulated kinase 1/2 （ERK 1/2） in rats with myocardial hypertrophy. Methods Totally 40 healthy Sprague-Dawley （SD） rats of clean grade were divided into 4 groups according to random digit table, i.e., the normal group, the model group, the electroa- cupuncture （EA） group, the sham-EA group, 10 in each group. Rats in the normal group were fed with reg- ular forage. Left ventricular myocardial hypertrophy model was established in rats of the rest 3 groups by subcutaneously injecting isoprinosine hydrochloride （ISO） （at the daily dose of 3 mg/kg） from the nape for a total of 14 days. Rats in the EA group were needed at Neiguan （PC6） using continuous wave （2 Hz, 1 mA, 20-min switching, once per day for 14 days）. Rats in the sham-EA group were needled at non-acu- points [5 mm from Neiguan （PC6）] in the same intervention method as the EA group. After intervention ECG was observed and body weight weighed in all rats. Their hearts were removed by open heart surgery and weighed after anesthesia, and then left ventricle were separated and weighed. At last heart weight in- dex （HWI） and left ventricular weight index （LVWl） were calculated. Protein contents of Raf-1, p-ERK 1/2, and ERK 1/2 in left ventricular myocardial tissue were detected by Western blot. Results Compared with the normal group, elevated ST-segment amplitude, HWl, LVWl, protein expressions of Raf-1 and p-ERK 1/2 were significantly higher in the model group with statistical significance （P 〈0.01 ）. Compared with the mod- el group and the sham-EA group, elevated ST-segment amplitude, HWI, LVWI, protein expressions of Raf- 1 and p-ERK 1/2 were significantly lower in the EA group with statistical significance （P 〈0.05）. Conclusion EA could effectively regulate myocardial ischemia in myocardial hypertrophy rats, reduce heart index, and lower protein expressions of Raf-1 and p-ERK 1/2, which might be one of signal regulating mechanisms for EA improving myocardial hypertrophy through Raf/MEK/ERK pathways.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2016年第11期1335-1339,共5页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家自然科学基金资助项目(No.81403460)
湖北中医药大学校级课题(No.2013-144
No.XJ2014KJ003)
关键词
心肌肥厚
电针
内关穴
原癌基因丝氨酸/苏氨酸蛋白激酶/丝裂原活化蛋白激酶/细胞外信号调节激酶
myocardial hypertrophy
electroacupuncture
Neiguan （PC6）
proto-oncogene serine/ threonine-protein kinase/mitogen-activated protein kinase kinase/extracellular signal-regulated kinase
