摘要
目的了解淮安地区流感病毒流行及变异特点。方法选择一个流感监测年度（2014-2015年）分离的流感病毒,进行HA1基因的扩增、测序,采用PHYLIP 3.6软件及popart1.7软件构建系统进化树及Network进化网络图,利用DnaSP5.1软件进行病毒多态性分析,利用MEGA 6.0软件进行氨基酸推导及相关特征分析。结果 2014-2015年淮安地区流行的流感病毒核苷酸同源性及多样度分别为98.2%～99.9%和0.009（新甲型H1N1）,97.3%～99.9%和0.010（H3N2）,98.7%～99.8%和0.009（B型Yamagata系）,其中B型流感Yamgata系为淮安地区优势株。新甲型H1N1流感病毒淮安株在进化树上形成一个独立的进化亚枝,由A/Beijing-Xingcheng-Nan-Pian/SWL11619分化形成;H3N2流感病毒淮安株形成3个进化亚枝,由A/HK/7409/2014辐射进化形成;B型Yamgata系流感病毒淮安株形成2个进化亚枝,由B/Zhejiang-Liandou/11000/2014或B/Jiangsu-Hailing/11443/2014分化形成。与疫苗株相比,淮安地区各型流感病毒均发生的变异：K180Q、S220T（新甲型H1N1）,N294K（H3N2）,H137Q、N144K（B型Yamagata系）。结论淮安地区一个流行年度有两次流感病毒流行高峰,与南方流行趋势一致。
Objective To study the epidemiological characteristics and variation of the influenza virus. Methods HA1 gene sequences recovered from Huai＇an isolates collected during the 2014-2015 influenza season were used to construct a phylogenetic tree and network diagram.Nucleotide polymorphisms of the influenza virus within a population were analyzed using DnaSP version 5.1.Amino acid sequences deduced from DNA were analyzed using MEGA5. Results During the 2014-2015 influenza season,the influenza virus present in Huai＇an had a nucleotide similarity to H1N1（pdm09）of 98.2%-99.9% and a diversity of 0.009,a nucleotide similarity to H3N2 of 97.3-99.9% and a diversity of 0.010,and a nucleotide similarity to the influenza B Yamagata lineage of 98.7-99.8%and a diversity of 0.009.In addition,the influenza B virus Yamagata lineage was the predominant virus in Huai＇an.Huai＇an isolates of the influenza H1N1（pdm09）virus clustered together and formed a genetic lineage originating from A/Beijing-Xingcheng-Nan-Pian/SWL11619.Huai＇an strains of the influenza H3N2 virus formed three lineages,with evolutionary branching from A/HK/7409/2014.Huai＇an strains of the influenza B virus Yamagata lineage originated from B/Zhejiang-Liandou/11000/2014 or B/Jiangsu-Hailing/11443/2014.Compared to vaccine strains,Huai＇an isolates of the influenza H1N1（pdm09）virus had K180 Qand S220T mutations,isolates of the influenza H3N2 virus had the N294 K mutation,and isolates of the influenza B virus Yamagata lineage had H137 Qand N144K mutations. Conclusion The prevalence of the influenza virus in Huai＇an peaked twice,coinciding with trends in southern China from 2014-2015.
出处
《中国病原生物学杂志》
CSCD
北大核心
2016年第9期788-794,798,共8页
Journal of Pathogen Biology
基金
国家传染病重大科技专项课题(No.2013ZX10004-101-006
2013ZX10004805-008)
淮安市科技局项目(No.HAS2015019-3)
淮安市预防医学会科研基金项目(No.hayf201516)
淮安市自然科学软课题(No.40)
关键词
淮安株
流感病毒
流行特征
分子进化
Huai＇an isolate
influenza virus
epidemiological characterization
molecular evolution
