摘要
米根霉脂肪酶(Rhizopus oryzae lipase)是一种具有广泛用途的工业化脂肪酶,对重组米根霉脂肪酶(r ProROL)的酶学性质进行考察,发现其水解甘油三酯时具有明显的sn-1,3位置专一性;催化二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)酯化时其活力分别为催化油酸酯化活力的37.42%和8%。鳀鱼油是天然EPA/DHA主要来源之一,在鳀鱼油中DHA含量为12.1%。13C核磁共振波谱分析表明,鳀鱼油中DHA主要分布在甘油酯的sn-2位,利用重组米根霉脂肪酶(r ProROL)催化鳀鱼油部分水解富集其中的DHA时,得到的1,2-甘油二酯中DHA的含量可以达到19.7%,甘油单酯中DHA的含量可以达到25.2%,DHA的回收率为65.6%。表明r ProROL脂肪酶具有水解鳀鱼油富集DHA的应用潜能。
Rhizopus oryzae lipase is one of the widely used industrial lipase. The enzymatic properties of recombinant rhizopus oryzae lipase were evaluated and it displayed strong 1,3 regiospecificity in hydroly- zing triglyceride; meanwhile, it showed 37.42% and 8.0% relative activity towards eicosapentaenoic acid（EPA） and docosahexenoic acid（DI-IA） compared with oleic acid in esterification reaction. Anchovy oil is one of the main sources of natural EPA/DHA and it contains 12.1% DHA. The result of 13C nu- clear magnetic resonance spectrum showed that DHA in anchovy oil was preferentially located at sn -2 position of the glyceride. Based on above, recombinant rhizopus oryzae lipase was employed to hydrolyze anchovy oil to concentrate DHA, and the contents of DHA in produced 1,2 - diacylglycerol and monoa- cylglycerol reached to 19.7% and 25.2% accompanying with a recovery yield of 65.6%. The results demonstrated that recombinant rhizopus oryzae lipase was a prospective lipase which could be used in en- richment of DHA by hydrolysis of anchovy oil.
作者
朱东奇
李道明
王卫飞
杨博
王永华
ZHU Dong - qi LI Dao - ming WANG Wei - fei YANG Bo WANG Yong - hua(College of Food Sciences and Engineering, South China University of Technology, Guangzhou Guangdong 510640 School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou Guangdong 510640 School of Bioscience and Bioengineering, South China University of Technology, Guangzhou Guangdong 510006)
出处
《粮油食品科技》
2016年第6期21-25,共5页
Science and Technology of Cereals,Oils and Foods
基金
国家高技术研究发展计划(863计划)(2014AA093601)
广东省产学研项目(2013B090200015)
关键词
重组米根霉脂肪酶
水解
鳀鱼油
DHA
recombinant rhizopus oryzae lipase
hydrolysis
anchovy oil
docosahexaenoic acid （DHA）
