摘要
目的探究紫花苜蓿黄酮在体外抑制肿瘤细胞活性的研究。方法采用四氮甲基唑蓝(MTT)法测定紫花苜蓿黄酮在体外的抗肿瘤活性;流式细胞术检测紫花苜蓿黄酮对人胃癌细胞MGC-803周期和凋亡的影响;Western blotting方法检测相关细胞蛋白的表达情况。结果 MGC-803细胞经紫花苜蓿黄酮预处理后,细胞存活率显著降低(P<0.05)。流式细胞术检测发现紫花苜蓿黄酮能够诱导细胞凋亡;引起细胞周期紊乱,使细胞阻滞在G0/G1期。Western blotting实验结果表明它能够活化促凋蛋白Caspase-9,Caspase-3,RARP,抑制抑凋蛋白Bcl-2的表达;引起相关细胞周期蛋白Cyclin D1,CDK4,CDK6的表达量下调。结论紫花苜蓿黄酮能够通过诱导MGC-803细胞凋亡途径和调节正常周期活动来抑制MGC-803细胞增殖,并且这种抑制作用还存在着时间和剂量依赖效应。
Objective To investigate the anticancer activity of in vitro of alfalfa flavonoids. flavones inhibit in vitro. Methods Antitumor activity of alfalfa flavonoids was measured by using MTT assay,the apoptosis and cell cycle distribution was using flow cytometry and the expression of relative proteins were analysed by Western blotting. Results The viability of MGC-803 cells decreased significantly（ P〈0. 05） after treated with alfalfa flavonoids,flow cytometry proved the apoptosis and disrupted cell cycle,caused cells arrested in G0/ G1 phase. Western blotting indicated that it activated Caspase-9,Caspase-3,RARP and inhibited Bcl-2; the cell cycle protein-Cyclin D1,CDK4 and CDK6 were expressed downregulated. Conclusion alfalfa flavonoids could inhibit the proliferation of MGC-803 cells by induction of apoptosis and disruption of cell cycle which still existed time-and dose-dependent effect.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2016年第9期2104-2106,共3页
Lishizhen Medicine and Materia Medica Research
基金
国家自然科学基金(No.31572430)
关键词
清肠化湿方
抗炎
镇痛
Alfalfa flavonoids
Human gastric cancer cells MGC-803
Apoptotic protein
Cyclins
