摘要
本文介绍了天然人白细胞γ-干扰素(HuIFN-γ)的部分纯化方法,用聚乙二醇4000(PEG4000)浓缩,硫酸铵沉淀和CM-Sephadex C-50纯化,可将粗制的HuIFN-γ纯化约100倍以上,效价达1.3×10~5u/ml,比活性为1.0×10~5u/mg蛋白.并进行了各项质量控制实验及10名正常人接受肌注纯化HuIFN-γ实验,体温、脉搏和血压未发现明显的变化,可供临床试用.
A method for partially purifying the native human gamma interferon (HuIFN-γ) was presented. The rude HuIFN-γwas concentrated by PEG 4 000, precipitated by (NH_4)_2SO_4 and purified by CM-Sephades C-50(more than 100 times). The titre of HuIFN-γwas 1.3X10^5u/ml and its specific activity was 1. 0 X 10~5u/mg protien. Quality control tests were performed and 10 volunteers received intramuscularly the purified HuIFN-γwithout significant changes in body temperature, pulse rate and blood pressure. It is considered to be suitable for clinical trial.
出处
《中国输血杂志》
CAS
CSCD
1991年第2期59-62,共4页
Chinese Journal of Blood Transfusion
